Transcriptional dynamics of transposable elements when converting fibroblast cells of Macaca mulatta to neuroepithelial stem cells

BMC Genomics

Junqiang Guo¹  Nan Zhou¹  Dahai Liu²  Xiaotuo Zhang³  Shipeng Li⁴  Kui Duan⁴  Li Liu⁴  Zhigang Zhao⁴  Yun Zheng⁵ 

[1] Faculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China;Foshan Stomatology Hospital and School of Medicine, Foshan University, Foshan, 528000, Guangdong, China;State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development Institute of Plant Biology, School of Life Sciences, Fudan University, 200438, Shanghai, China;State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China;State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China;Faculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China;

关键词: Transposable element (TE);    Endogenous retrovirus (ERV);    Long terminal repeat (LTR);    Transcription;    Fibroblast;    Induced pluripotent stem cell (iPSC);    Neuroepithelial stem cell (NESC);   

DOI  :  10.1186/s12864-021-07717-9

来源: Springer

【 摘 要 】

BackgroundTransposable elements (TE) account for more than 50% of human genome. It has been reported that some types of TEs are dynamically regulated in the reprogramming of human cell lines. However, it is largely unknown whether some TEs in Macaca mulatta are also regulated during the reprogramming of cell lines of monkey.ResultsHere, we systematically examined the transcriptional activities of TEs during the conversion of Macaca mulatta fibroblast cells to neuroepithelial stem cells (NESCs). Hundreds of TEs were dynamically regulated during the reprogramming of Macaca mulatta fibroblast cells. Furthermore, 48 Long Terminal Repeats (LTRs), as well as some integrase elements, of Macaca endogenous retrovirus 3 (MacERV3) were transiently activated during the early stages of the conversion process, some of which were further confirmed with PCR experiments. These LTRs were potentially bound by critical transcription factors for reprogramming, such as KLF4 and ETV5.ConclusionThese results suggest that the transcription of TEs are delicately regulated during the reprogramming of Macaca mulatta fibroblast cells. Although the family of ERVs activated during the reprogramming of fibroblast cells in Macaca mulatta is different from those in the reprogramming of human fibroblast cells, our results suggest that the activation of some ERVs is a conserved mechanism in primates for converting fibroblast cells to stem cells.

【 授权许可】

CC BY