Studies of drug interactions with glycated human serum albumin by high-performance affinity chromatography

Reviews in analytical chemistry

article

Ryan Matsuda¹  So-Hwang Kye¹  Jeanethe Anguizola¹  David S. Hage¹ 

[1] Department of Chemistry, University of Nebraska

关键词: binding studies;    diabetes;    drug-protein binding;    human serum albumin;    glycation;    high-performance affinity chromatography;    sulfonylurea drugs;   

DOI  :  10.1515/revac-2013-0029

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

Diabetes is a health condition associated with the elevated levels of glucose in the bloodstream and affects 366 million people worldwide. Type II diabetes is often treated with sulfonylurea drugs, which are known to bind tightly in the blood to the transport protein human serum albumin (HSA). One consequence of the elevated levels of glucose in diabetes is the nonenzymatic glycation of proteins such as HSA. Several areas of HSA are now known to be affected by glycation-related modifications, which may in turn affect the binding of sulfonylurea drugs and other solutes to this protein. This review discusses some recent studies that have examined these changes in drug-protein binding by employing high-performance affinity chromatography (HPAC). A description of the theoretical and experimental techniques that were used in these studies is given. The information on drug interactions with glycated HSA, as obtained through this method, is also summarized. In addition, the potential advantages of this approach in the areas of biointeraction analysis and personalized medicine are considered.

【 授权许可】

CC BY