High-efficiency, large-area lattice light-sheet generation by dielectric metasurfaces

Nanophotonics

article

Fenghua Shi¹  Jing Wen²  Dangyuan Lei³ 

[1] Anhui Province Key Laboratory of Optoelectric Materials Science and Technology, College of Physics and Electronic Information, Anhui Normal University;Engineering Research Center of Optical Instrument and Systems, Ministry of Education and Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology;Department of Materials Science and Engineering, City University of Hong Kong

关键词: field of view;    illumination efficiency;    lattice light-sheet microscopy;    optical metasurface;    spatial frequency manipulation;   

DOI  :  10.1515/nanoph-2020-0227

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

Lattice light-sheet microscopy (LLSM) was developed for long-term live-cell imaging with ultra-fine three-dimensional (3D) spatial resolution, high temporal resolution, and low photo-toxicity by illuminating the sample with a thin lattice-like light-sheet. Currently available schemes for generating thin lattice light-sheets often require complex optical designs. Meanwhile, limited by the bulky objective lens and optical components, the light throughput of existing LLSM systems is rather low. To circumvent the above problems, we utilize a dielectric metasurface of a single footprint to replace the conventional illumination modules used in the conventional LLSM and generate a lattice light-sheet with a ~3-fold broader illumination area and a significantly leveraged illumination efficiency, which consequently leads to a larger field of view with a higher temporal resolution at no extra cost of the spatial resolution. We demonstrate that the metasurface can manipulate spatial frequencies of an input laser beam in orthogonal directions independently to break the trade-off between the field of view and illumination efficiency of the lattice light-sheet. Compared to the conventional LLSM, our metasurface module serving as an ultra-compact illumination component for LLSM at an ease will potentially enable a finer spatial resolution with a larger numerical-aperture detection objective lens.

【 授权许可】

CC BY