Enhanced electric field sensitivity of quantum dot/rod two-photon fluorescence and its relevance for cell transmembrane voltage imaging

Nanophotonics

article

Stijn Jooken¹  Zeger Hens²  Dirk Dorfs³  Thierry Verbiest²  Koen Clays²  Geert Callewaert⁴  Carmen Bartic¹  Yovan de Coene²  Olivier Deschaume¹  Dániel Zámbó³  Tangi Aubert² 

[1] Department of Physics and Astronomy;Department of Chemistry, Ghent University;Institute of Physical Chemistry and Electrochemistry, Leibniz Universität Hannover;Department of Cellular and Molecular Medicine, KU Leuven Campus Kulak;University of Montpellier

关键词: electric field;    quantum dot/rod;    semiconductor nanoparticle;    two-photon fluorescence;    voltage sensing;   

DOI  :  10.1515/nanoph-2021-0077

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

The optoelectronic properties of semiconductor nanoparticles make them valuable candidates for the long-term monitoring of transmembrane electric fields in excitable cells. In this work, we show that the electric field sensitivity of the fluorescence intensity of type-I and quasi-type-II quantum dots and quantum rods is enhanced under two-photon excitation compared to single-photon excitation. Based on the superior electric field sensitivity of the two-photon excited fluorescence, we demonstrate the ability of quantum dots and rods to track fast switching E-fields. These findings indicate the potential of semiconductor nanoparticles as cellular voltage probes in multiphoton imaging.

【 授权许可】

CC BY