Investigation of mixture toxicity of widely used drugs caffeine and ampicillin in the presence of an ACE inhibitor on bacterial growth using droplet-based microfluidic technique

Green Processing and Synthesis

article

Jialan Cao¹  Juliane Goldhan¹  Karin Martin²  J. Michael Köhler¹ 

[1] Institute of Chemistry and Biotechnology, Department of Physical Chemistry/Microreaction Technology, Technical University of Ilmenau;Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute

关键词: ACE inhibitor captopril;    antibiotic ampicillin;    combinatorial effects;    droplet-based microfluidic technique;    mixture toxicity;   

DOI  :  10.1515/gps-2013-0078

来源: De Gruyter

【 摘 要 】

Droplet-based microfluidic technique is very suitable for a variety of screening processes. The cultivation within nanoliter segments and multidimensional microtoxicological screening of the Gram-negative bacterium Escherichia coli were studied under droplet-based microfluidic conditions. In order to evaluate the toxicity of the binary and ternary mixtures of antibiotic ampicillin and caffeine in the presence of the angiotensin-converting enzyme inhibitor captopril, a time-resolved optical double endpoint detection unit was applied. It included a microflow-through fluorimeter and photometer, which can simultaneously analyze changes in the endogenous autofluorescence signal and the cell density of E. coli cultivated inside 450-nl microfluid segments. As a result, strong nonlinear combination effects and a concentration-dependent antagonistic effect, as well as formation of activity summits on bolographic maps, were found. Our findings confirm the importance of multiparameter investigations for toxicological studies and could be taken into account in medical practice.

【 授权许可】

CC BY