NADH oxidation in a microreactor catalysed by ADH immobilised on γ -Fe 2 O 3 nanoparticles

Green Processing and Synthesis

article

Anita Šalić¹  Katarina Pindrić¹  Gordana Hojnik Podrepšek²  Maja Leitgeb²  Bruno Zelić¹ 

[1] Faculty of Chemical Engineering and Technology, University of Zagreb;Faculty of Chemistry and Chemical Engineering, University of Maribor

关键词: alcohol dehydrogenase;    coenzyme regeneration;    enzyme immobilisation;    microreactor;    maghemite nanoparticle;   

DOI  :  10.1515/gps-2013-0084

来源: De Gruyter

【 摘 要 】

A new concept of nicotinamide adenine dinucleotide hydrate (NADH) oxidation which combines advantages of the microreactor technology with the advantages of magnetic nanoparticles (MNPs) application is developed. Acetaldehyde was used as a substrate for the NADH regeneration process while the reaction was performed in a batch reactor and in a microreactor using alcohol dehydrogenase (ADH)-loaded MNPs. Three different microreactor systems with MNPs were studied, two with stationary MNPs trapped on the inner surface of microchannel by permanent magnetic field and one where the MNPs actively moved across the channel (movement inside microchannel allowed by an oscillating magnetic field). In a reactor system with an oscillating magnetic field and an actively moving ADH-loaded MNPs 100% NADH conversion was achieved for residence time of just 2 min.

【 授权许可】

CC BY