期刊论文详细信息
Molecules
Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis
Hui Lin1  Meijing Sun1  Zewang Guo1  Liaoyuan Zhang1  Wensong Jin1  Yunlong Yang1  Jun Yuan1  Feixue Chen1  Yuanzhi He1  Huifang Gao1  Jiebo Chen2 
[1] Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China;National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
关键词: meso-2,3-butanediol;    (3S)-acetoin;    (2S,3S)-butanediol;    Escherichia coli;    coenzyme regeneration;    whole-cell biocatalysis;   
DOI  :  10.3390/molecules23030691
来源: DOAJ
【 摘 要 】

(3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of (3S)-acetoin and (2S,3S)-2,3-butanediol from meso-2,3-butanediol. First, E. coli co-expressing (2R,3R)-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin was developed for (3S)-acetoin production from meso-2,3-butanediol. Maximum (3S)-acetoin concentration of 72.38 g/L with the stereoisomeric purity of 94.65% was achieved at 24 h under optimal conditions. Subsequently, we developed another biocatalyst co-expressing (2S,3S)-2,3-butanediol dehydrogenase and formate dehydrogenase for (2S,3S)-2,3-butanediol production from (3S)-acetoin. Synchronous catalysis together with two biocatalysts afforded 38.41 g/L of (2S,3S)-butanediol with stereoisomeric purity of 98.03% from 40 g/L meso-2,3-butanediol. These results exhibited the potential for (3S)-acetoin and (2S,3S)-butanediol production from meso-2,3-butanediol as a substrate via whole-cell biocatalysis.

【 授权许可】

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