Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis

Molecules

Hui Lin¹  Meijing Sun¹  Zewang Guo¹  Liaoyuan Zhang¹  Wensong Jin¹  Yunlong Yang¹  Jun Yuan¹  Feixue Chen¹  Yuanzhi He¹  Huifang Gao¹  Jiebo Chen² 

[1] Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China;National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

关键词: meso-2,3-butanediol;    (3S)-acetoin;    (2S,3S)-butanediol;    Escherichia coli;    coenzyme regeneration;    whole-cell biocatalysis;   

DOI  :  10.3390/molecules23030691

来源: DOAJ

【 摘 要 】

(3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of (3S)-acetoin and (2S,3S)-2,3-butanediol from meso-2,3-butanediol. First, E. coli co-expressing (2R,3R)-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin was developed for (3S)-acetoin production from meso-2,3-butanediol. Maximum (3S)-acetoin concentration of 72.38 g/L with the stereoisomeric purity of 94.65% was achieved at 24 h under optimal conditions. Subsequently, we developed another biocatalyst co-expressing (2S,3S)-2,3-butanediol dehydrogenase and formate dehydrogenase for (2S,3S)-2,3-butanediol production from (3S)-acetoin. Synchronous catalysis together with two biocatalysts afforded 38.41 g/L of (2S,3S)-butanediol with stereoisomeric purity of 98.03% from 40 g/L meso-2,3-butanediol. These results exhibited the potential for (3S)-acetoin and (2S,3S)-butanediol production from meso-2,3-butanediol as a substrate via whole-cell biocatalysis.

【 授权许可】

Unknown