Ultrasound assisted three phase partitioning of peroxidase from waste orange peels

Green Processing and Synthesis

article

Mangesh D. Vetal¹  Virendra K. Rathod¹ 

[1] Institute of Chemical Technology, Department of Chemical Engineering

关键词: Citrus sinensis;    extraction;    peroxidase;    ultrasound assisted three phase partitioning;   

DOI  :  10.1515/gps-2015-0116

来源: De Gruyter

【 摘 要 】

Ultrasound assisted three phase partitioning (UATPP) has been explored to intensify the extraction and purification of peroxidase from orange peel ( Citrus sinensis ). UATPP of peroxidase was studied by varying different process parameters such as ammonium sulfate concentration, pH, broth to tertiary butanol (t-butanol) ratio, temperature, ultrasound frequency, ultrasound power and duty cycle. Under optimized conditions, i.e. 50% ammonium sulfate saturation, pH 6, temperature 30°C, broth to t-butanol ratio 1:1.5 (v/v), at 25 kHz frequency and 150 W ultrasonication power with 40% duty cycle, 6 min irradiation time, 24.28 fold purity of peroxidase with 91.84% recovery was been obtained. UATPP resulted in higher purification fold of peroxidase due to enhanced mass transfer with reduced operation time from 80 min to 6 min as compared to conventional three phase partitioning (TPP). SDS PAGE analysis of the partially purified enzyme indicated a molecular weight of 26 kDa. The integration of ultrasound with TPP enhances the utility of both processes. Thus, UATPP has been proved as an efficient alternative to partial purification of peroxidase.

【 授权许可】

CC BY