Reduction of protein kinase C α (PKC‐α) promote apoptosis via down‐regulation of Dicer in bladder cancer

Journal of Cellular and Molecular Medicine

Zhenming Jiang¹  Chuize Kong¹  Zhe Zhang¹  Yuyan Zhu¹  Yuxi Zhang¹ 

[1] Department of Urology, The First Affiliated Hospital of China Medical University, Shenyang, China

关键词: PKC‐α;    Dicer;    apoptosis;    bladder cancer;    caspase‐3;   

DOI  :  10.1111/jcmm.12503

来源: Wiley

【 摘 要 】

Abstract

In clinic, we examined the expression of protein kinase C (PKC)-α and Dicer in the samples of bladder cancer patients, and found that the two proteins have a line correlation. Our study confirmed this correlation existing by clearing the decreasing expression of Dicer after the PKC-α knockdown. Treatment of bladder cancer cell lines (T24, 5637) with the PKC-α or Dicer knockdown and the PKC inhibitors (Calphostin C and Gö 6976) can promote the apoptosis. Inhibition of PKC can increase the activities of caspase-3 and PARP, however, decrease the expression of Dicer. And knockdown of the PKC-α or Dicer can also activate the caspase-3 or the PARP. Considering the reduction of PKC-α can induce the Dicer down-regulation, we make the conclusion that the reduction of PKC-α can promote the apoptosis via the down-regulation of Dicer in bladder cancer.

【 授权许可】

CC BY   
© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

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