Reprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress

Journal of Cellular and Molecular Medicine

Karina D. Asensi¹  Rodrigo S. Fortunato¹  Danúbia S. dos Santos¹  Thaísa S. Pacheco¹  Danielle F. de Rezende¹  Deivid C. Rodrigues¹  Fernanda C. P. Mesquita¹  Tais H. Kasai-Brunswick¹  Antonio C. Campos de Carvalho¹  Denise P. Carvalho¹  Adriana B. Carvalho¹ 

[1] Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

关键词: induced pluripotent stem cells;    mesenchymal stem cells;    oxidative stress;    reactive oxygen species;    menstrual blood;   

DOI  :  10.1111/jcmm.12226

来源: Wiley

【 摘 要 】

Abstract

Properties of induced pluripotent stem cells (iPSC) have been extensively studied since their first derivation in 2006. However, the modification in reactive oxygen species (ROS) production and detoxification caused by reprogramming still needs to be further elucidated. The objective of this study was to compare the response of iPSC generated from menstrual blood–derived mesenchymal stem cells (mb-iPSC), embryonic stem cells (H9) and adult menstrual blood–derived mesenchymal stem cells (mbMSC) to ROS exposure and investigate the effects of reprogramming on cellular oxidative stress (OS). mbMSC were extremely resistant to ROS exposure, however, mb-iPSC were 10-fold less resistant to H₂O₂, which was very similar to embryonic stem cell sensitivity. Extracellular production of ROS was also similar in mb-iPSC and H9 and almost threefold lower than in mbMSC. Furthermore, intracellular amounts of ROS were higher in mb-iPSC and H9 when compared with mbMSC. As the ability to metabolize ROS is related to antioxidant enzymes, we analysed enzyme activities in these cell types. Catalase and superoxide dismutase activities were reduced in mb-iPSC and H9 when compared with mbMSC. Finally, cell adhesion under OS conditions was impaired in mb-iPSC when compared with mbMSC, albeit similar to H9. Thus, reprogramming leads to profound modifications in extracellular ROS production accompanied by loss of the ability to handle OS.

【 授权许可】

CC BY   
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.