Telocytes in human oesophagus

Journal of Cellular and Molecular Medicine

Xiaoke Chen¹  Yonghua Zheng²  Catalin G. Manole⁴  Xiangdong Wang³ 

[1] Department of thoracic surgery, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Respiratory Medicine, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China;Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Cellular and Molecular Medicine, ‘Carold Davila’ University of Medicine and Pharmacy, Bucharest, Romania

关键词: telocytes;    telopodes;    human oesophagus;    VEGF;    EGF;    stromal synapses;    tissue repair;   

DOI  :  10.1111/jcmm.12149

来源: Wiley

【 摘 要 】

Abstract

Telocytes (TCs), a new type of interstitial cells, were identified in many different organs and tissues of mammalians and humans. In this study, we show the presence, in human oesophagus, of cells having the typical features of TCs in lamina propria of the mucosa, as well as in muscular layers. We used transmission electron microscopy (TEM), immunohistochemistry (IHC) and primary cell culture. Human oesophageal TCs present a small cell body with 2–3 very long Telopodes (Tps). Tps consist of an alternation of thin segments (podomers) and thick segments (podoms) and have a labyrinthine spatial arrangement. Tps establish close contacts (‘stromal synapses’) with other neighbouring cells (e.g. lymphocytes, macrophages). The ELISA testing of the supernatant of primary culture of TCs indicated that the concentrations of VEGF and EGF increased progressively. In conclusion, our study shows the existence of typical TCs at the level of oesophagus (mucosa, submucosa and muscular layer) and suggests their possible role in tissue repair.

【 授权许可】

CC BY   
© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.