The therapeutic effect of Rosuvastatin on cardiac remodelling from hypertrophy to fibrosis during the end‐stage hypertension in rats

Journal of Cellular and Molecular Medicine

W.B. Zhang²  Q.J. Du²  H. Li²  A.J. Sun²  Z.H. Qiu²  C.N. Wu²  G. Zhao²  H. Gong¹  K. Hu²  Y.Z. Zou¹ 

[1] Institute of Biomedical Science, Fudan University, Shanghai, China;Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China

关键词: old‐aged spontaneously hypertensive rats;    cardiac remodelling;    hypertrophy;    fibrosis;    apoptosis;    Rosuvastatin;    3‐hydroxy‐3‐;   

DOI  :  10.1111/j.1582-4934.2012.01536.x

来源: Wiley

【 摘 要 】

End-stage hypertensive heart disease is an increasing cause of cardiac mortality. Therefore, the current study focused on the cardiac remodelling from hypertrophy to fibrosis in old-aged spontaneously hypertensive rats (SHRs), and explored the therapeutic effects of Rosuvastatin and its possible mechanism(s) of action. Spontaneously hypertensive rats at age 52 weeks were randomly divided into three groups, the first two to receive Rosuvastatin at a dose of 20 mg/kg/day and 40 mg/kg/day, respectively, and the third to receive placebo, which was to be compared with Wistar-Kyoto as controls. After 2-month treatment, SBP, heart to body weight ratio (HW/BW%) and echocardiographic features were evaluated, followed by haematoxylin and eosin and Masson trichrome staining in conjunction with qPCR of foetal gene expressions. Transferase-mediated dUTP nick-end labelling assay and immunofluorescent labelling for active caspase-3 were used to detect the apoptotic cardiomyocytes. Signaling pathways involved were examined by using western blot. Old-aged SHR developed end-stage hypertensive heart disease characterized by significant enhancement of HW/BW%, LVAWd and LVPWd, and decreased LVEF and LVFS, accompanied by cardiomyocytes enlargement and fibrosis along with activation of foetal gene programme. Cardiac apoptosis increased significantly during the transition process.

【 授权许可】

Unknown   
© 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd