Expression of nitrous oxide reductase from Pseudomonas stutzeri in transgenic tobacco roots using the root‐specific rolD promoter from Agrobacterium rhizogenes

Ecology and Evolution

Shen Wan¹  Amanda M. Johnson¹ 

[1] Department of Biochemistry, Microbiology and Immunology, Center for Research on Environmental Microbiology – CREM, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, K1H 8M5, Canada

关键词: Greenhouse gas;    nitrous oxide;    nitrous oxide reductase;    phytoremediation;    rhizosecretion;    root‐specific expression;   

DOI  :  10.1002/ece3.74

来源: Wiley

【 摘 要 】

Abstract

The nitrous oxide (N₂O) reduction pathway from a soil bacterium, Pseudomonas stutzeri, was engineered in plants to reduce N₂O emissions. As a proof of principle, transgenic plants expressing nitrous oxide reductase (N₂OR) from P. stutzeri, encoded by the nosZ gene, and other transgenic plants expressing N₂OR along with the more complete operon from P. stutzeri, encoded by nosFLZDY, were generated. Gene constructs were engineered under the control of a root-specific promoter and with a secretion signal peptide. Expression and rhizosecretion of the transgene protein were achieved, and N₂OR from transgenic Nicotiana tabacum proved functional using the methyl viologen assay. Transgenic plant line 1.10 showed the highest specific activity of 16.7 µmol N₂O reduced min⁻¹ g⁻¹ root protein. Another event, plant line 1.9, also demonstrated high specific activity of N₂OR, 13.2 µmol N₂O reduced min⁻¹ g⁻¹ root protein. The availability now of these transgenic seed stocks may enable canopy studies in field test plots to monitor whole rhizosphere N flux. By incorporating one bacterial gene into genetically modified organism (GMO) crops (e.g., cotton, corn, and soybean) in this way, it may be possible to reduce the atmospheric concentration of N₂O that has continued to increase linearly (about 0.26% year⁻¹) over the past half-century.

【 授权许可】

CC BY-NC   
© 2011 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.

Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.