Patterns of differential gene expression in adult rotation‐resistant and wild‐type western corn rootworm digestive tracts

Evolutionary Applications

Chia-Ching Chu²  Jorge A. Zavala⁵  Joseph L. Spencer¹  Matías J. Curzi³  Christopher J. Fields⁶  Jenny Drnevich⁶  Blair D. Siegfried⁴ 

[1] Illinois Natural History Survey, University of Illinois, Champaign, IL, USA;Department of Crop Sciences, University of Illinois, Urbana, IL, USA;DuPont Pioneer, Salto, Buenos Aires, Argentina;Department of Entomology, University of Nebraska, Lincoln, NE, USA;Facultad de Agronomía, Cátedra de Bioquímica INBA-CONICET, University of Buenos Aires-CONICET, Buenos Aires, Argentina;High-Performance Biological Computing, Roy J. Carver Biotechnology Center, Urbana, IL, USA

关键词: adaptation;    agriculture;    ecological genetics;   

DOI  :  10.1111/eva.12278

来源: Wiley

【 摘 要 】

Abstract

The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn. Annual crop rotation between corn and soybean disrupts the corn-dependent WCR life cycle and is widely adopted to manage this pest. This strategy selected for rotation-resistant (RR) WCR with reduced ovipositional fidelity to corn. Previous studies revealed that RR-WCR adults exhibit greater tolerance of soybean diets, different gut physiology, and host–microbe interactions compared to rotation-susceptible wild types (WT). To identify the genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA-sequencing analyses of RNA libraries from different WCR phenotypes fed with corn or soybean diets. Global gene expression profiles of WT- and RR-WCR were similar when feeding on corn diets, but different when feeding on soybean. Using network-based methods, we identified gene modules transcriptionally correlated with the RR phenotype. Gene ontology enrichment analyses indicated that the functions of these modules were related to metabolic processes, immune responses, biological adhesion, and other functions/processes that appear to correlate to documented traits in RR populations. These results suggest that gut transcriptomic divergence correlated with brief soybean feeding and other physiological traits may exist between RR- and WT-WCR adults.

【 授权许可】

CC BY   
© 2015 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.