Molecular Systems Biology | |
A dynamical model of the spindle position checkpoint | |
Ayse Koca Caydasi1  Maiko Lohel1  Gerd Grünert2  Peter Dittrich2  Gislene Pereira1  | |
[1] Molecular Biology of Centrosomes and Cilia, German Cancer Research Center, DKFZ-ZMBH Alliance, Heidelberg, Germany;Bio Systems Analysis Group, Institute of Computer Science, Jena Centre for Bioinformatics and Friedrich Schiller University, Jena, Germany | |
关键词: Bfa1; mitotic exit network; modeling and simulation; spindle position checkpoint; Tem1; | |
DOI : 10.1038/msb.2012.15 | |
来源: Wiley | |
【 摘 要 】
The orientation of the mitotic spindle with respect to the polarity axis is crucial for the accuracy of asymmetric cell division. In budding yeast, a surveillance mechanism called the spindle position checkpoint (SPOC) prevents exit from mitosis when the mitotic spindle fails to align along the mother-to-daughter polarity axis. SPOC arrest relies upon inhibition of the GTPase Tem1 by the GTPase-activating protein (GAP) complex Bfa1–Bub2. Importantly, reactions signaling mitotic exit take place at yeast centrosomes (named spindle pole bodies, SPBs) and the GAP complex also promotes SPB localization of Tem1. Yet, whether the regulation of Tem1 by Bfa1–Bub2 takes place only at the SPBs remains elusive. Here, we present a quantitative analysis of Bfa1–Bub2 and Tem1 localization at the SPBs. Based on the measured SPB-bound protein levels, we introduce a dynamical model of the SPOC that describes the regulation of Bfa1 and Tem1. Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition. The spindle position checkpoint (SPOC) is an important surveillance mechanism in the budding yeast cell cycle. An integrated approach, combining quantitative experimental cell biology with mathematical modeling, reveals how the SPOC inhibits mitotic exit at the molecular level.Abstract
Synopsis
【 授权许可】
CC BY-NC-ND
Copyright © 2012 EMBO and Macmillan Publishers Limited
Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
【 预 览 】
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