RNAi screen of Salmonella invasion shows role of COPI in membrane targeting of cholesterol and Cdc42

Molecular Systems Biology

Benjamin Misselwitz¹  Sabrina Dilling¹  Pascale Vonaesch¹  Raphael Sacher³  Berend Snijder³  Markus Schlumberger¹  Samuel Rout¹  Manuel Stark²  Christian von Mering²  Lucas Pelkmans³ 

[1] Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland;Institute of Molecular Life Sciences, University of Zürich, Zürich, Switzerland;Institute of Molecular Systems Biology, D-BIOL, ETH Zürich, Zürich, Switzerland

关键词: coatomer;    HeLa;    Salmonella;    siRNA;    systems biology;   

DOI  :  10.1038/msb.2011.7

来源: Wiley

【 摘 要 】

The pathogen Salmonella Typhimurium is a common cause of diarrhea and invades the gut tissue by injecting a cocktail of virulence factors into epithelial cells, triggering actin rearrangements, membrane ruffling and pathogen entry. One of these factors is SopE, a G-nucleotide exchange factor for the host cellular Rho GTPases Rac1 and Cdc42. How SopE mediates cellular invasion is incompletely understood. Using genome-scale RNAi screening we identified 72 known and novel host cell proteins affecting SopE-mediated entry. Follow-up assays assigned these ‘hits’ to particular steps of the invasion process; i.e., binding, effector injection, membrane ruffling, membrane closure and maturation of the Salmonella-containing vacuole. Depletion of the COPI complex revea

【 授权许可】

CC BY-NC-SA   
Copyright © 2011 EMBO and Macmillan Publishers Limited

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