Toward an understanding of the protein interaction network of the human liver

Molecular Systems Biology

Jian Wang¹  Keke Huo²  Lixin Ma³  Liujun Tang¹  Dong Li¹  Xiaobi Huang¹  Yanzhi Yuan¹  Chunhua Li³  Wei Wang²  Wei Guan¹  Hui Chen¹  Chaozhi Jin¹  Junchen Wei¹  Wanqiao Zhang¹  Yongsheng Yang¹  Qiongming Liu¹  Ying Zhou¹  Cuili Zhang¹  Zhihao Wu¹  Wangxiang Xu¹  Ying Zhang¹  Tao Liu¹  Donghui Yu¹  Yaping Zhang¹  Liang Chen³  Dewu Zhu³  Xing Zhong³  Lixin Kang³  Xiang Gan³  Xiaolan Yu³  Qi Ma²  Jing Yan²  Li Zhou²  Zhongyang Liu¹  Yunping Zhu¹  Tao Zhou⁴  Fuchu He¹ 

[1] State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China;State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China;Institute of Biochemistry and Molecular Biology, Hubei University, Wuhan, China;National Center of Biomedical Analysis, Beijing, China

关键词: human liver;    network;    protein–protein interaction;    yeast two hybrid;   

DOI  :  10.1038/msb.2011.67

来源: Wiley

【 摘 要 】

Abstract

Proteome-scale protein interaction maps are available for many organisms, ranging from bacteria, yeast, worms and flies to humans. These maps provide substantial new insights into systems biology, disease research and drug discovery. However, only a small fraction of the total number of human protein–protein interactions has been identified. In this study, we map the interactions of an unbiased selection of 5026 human liver expression proteins by yeast two-hybrid technology and establish a human liver protein interaction network (HLPN) composed of 3484 interactions among 2582 proteins. The data set has a validation rate of over 72% as determined by three independent biochemical or cellular assays. The network includes metabolic enzymes and liver-specific, liver-phenotype and liver-disease proteins that are individually critical for the maintenance of liver functions. The liver enriched proteins had significantly different topological properties and increased our understanding of the functional relationships among proteins in a liver-specific manner. Our data represent the first comprehensive description of a HLPN, which could be a valuable tool for understanding the functioning of the protein interaction network of the human liver.

Synopsis

An extensive interaction network of human liver-expressed proteins is described, composed of 3484 interactions among 2582 proteins. Proteins associated with liver disease tend to be central and highly connected in the network.

• The interactions of a broad selection of human liver-expressed proteins were mapped by yeast two-hybrid technology, establishing a human liver protein interaction network composed of 3484 interactions among 2582 proteins.
• The data represent the first comprehensive description and analysis of the human liver protein interaction network.
• Proteins with known associations with liver diseases or phenotypes tend to be central and highly connected in the network, while liver-specific proteins and metabolic enzymes are preferentially connected to central proteins.

【 授权许可】

CC BY-NC-SA   
Copyright © 2011 EMBO and Macmillan Publishers Limited

Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.