Cellular reprogramming by the conjoint action of ERα, FOXA1, and GATA3 to a ligand‐inducible growth state

Molecular Systems Biology

Say Li Kong²  Guoliang Li¹  Siang Lin Loh²  Wing-Kin Sung¹ 

[1] Computational and Mathematical Biology, Genome Institute of Singapore, Singapore;Cancer Biology and Pharmacology 2, Genome Institute of Singapore, Singapore

关键词: enhanceosome;    estrogen receptor α;    FOXA1;    GATA3;    synthetic phenotypes;   

DOI  :  10.1038/msb.2011.59

来源: Wiley

【 摘 要 】

Despite the role of the estrogen receptor α (ERα) pathway as a key growth driver for breast cells, the phenotypic consequence of exogenous introduction of ERα into ERα-negative cells paradoxically has been growth inhibition. We mapped the binding profiles of ERα and its interacting transcription factors (TFs), FOXA1 and GATA3 in MCF-7 breast carcinoma cells, and observed that these three TFs form a functional enhanceosome that regulates the genes driving core ERα function and cooperatively modulate the transcriptional networks previously ascribed to ERα alone. We demonstrate that these enhanceosome occupied sites are associated with optimal enhancer characteristics with highest p300 co-activator recruitment, RNA Pol II occupancy, and chromatin o

【 授权许可】

CC BY-NC-ND   
Copyright © 2011 EMBO and Macmillan Publishers Limited

Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.