Adaptation by stochastic switching of a monostable genetic circuit in Escherichia coli

Molecular Systems Biology

Saburo Tsuru¹  Nao Yasuda²  Yoshie Murakami¹  Junya Ushioda¹  Akiko Kashiwagi³  Shingo Suzuki¹  Kotaro Mori²  Bei-Wen Ying¹ 

[1] Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Osaka, Japan;Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan;Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan

关键词: adaptation;    gene regulation;    monostability;    stochastic switching;    transciptome;   

DOI  :  10.1038/msb.2011.24

来源: Wiley

【 摘 要 】

Stochastic switching is considered as a cost-saving strategy for adaptation to environmental challenges. We show here that stochastic switching of a monostable circuit can mediate the adaptation of the engineered OSU12-hisC Escherichia coli strain to histidine starvation. In this strain, the hisC gene was deleted from the His operon and placed under the control of a monostable foreign promoter. In response to histidine depletion, the OSU12-hisC population shifted to a higher HisC expression level, which is beneficial under starving conditions but is not favoured by the monostable circuit. The population shift was accompanied by growth recovery and was reversible upon histidine addition. A weak directionality in stochastic switching of hisC wa

【 授权许可】

CC BY-NC-SA   
Copyright © 2011 EMBO and Macmillan Publishers Limited

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