Genomovar assignment of Pseudomonas stutzeri populations inhabiting produced oil reservoirs

MicrobiologyOpen

Fan Zhang⁴  Yue-Hui She³  Ibrahim M. Banat¹  Lu-Jun Chai⁴  Liu-Qin Huang²  Shao-Jin Yi³  Zheng-Liang Wang³  Hai-Liang Dong² 

[1] Faculty of Life and Health Sciences, University of Ulster, Coleraine, N. Ireland, U.K;State Key Laboratory of Biogeology and Environmental Microbiology, University of Geosciences (Beijing), Beijing, China;College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, China;The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, China

关键词: 16S rRNA gene;    Genomovar;    oil reservoirs;    Pseudomonas stutzeri;   

DOI  :  10.1002/mbo3.179

来源: Wiley

【 摘 要 】

Abstract

Oil reservoirs are specific habitats for the survival and growth of microorganisms in general. Pseudomonas stutzeri which is believed to be an exogenous organism inoculated into oil reservoirs during the process of oil production was detected frequently in samples from oil reservoirs. Very little is known, however, about the distribution and genetic structure of P. stutzeri in the special environment of oil reservoirs. In this study, we collected 59 P. stutzeri 16S rRNA gene sequences that were identified in 42 samples from 25 different oil reservoirs and we isolated 11 cultured strains from two representative oil reservoirs aiming to analyze the diversity and genomovar assignment of the species in oil reservoirs. High diversity of P. stutzeri was observed, which was exemplified in the detection of sequences assigned to four known genomovars 1, 2, 3, 20 and eight unknown genomic groups of P. stutzeri. The frequent detection and predominance of strains belonging to genomovar 1 in most of the oil reservoirs under study indicated an association of genomovars of P. stutzeri with the oil field environments.

【 授权许可】

CC BY   
© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

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