Characterization of a bacterial tyrosine kinase in Porphyromonas gingivalis involved in polymicrobial synergy

MicrobiologyOpen

Christopher J. Wright²  Peng Xue¹  Takanori Hirano⁴  Chengcheng Liu²  Sarah E. Whitmore²  Murray Hackett³ 

[1] Key Laboratory of Molecular and Cellular Immunology, Henan University, Kaifeng, China;Oral Health and Systemic Disease, University of Louisville, Louisville, Kentucky;Department of Chemical Engineering and Center for Microbial Proteomics, University of Washington, Seattle, Washington;University of Florida, Department of Oral Biology, Gainesville, Florida

关键词: Community;    periodontitis;    polymicrobial synergy;    Porphyromonas gingivalis;    tyrosine kinase;   

DOI  :  10.1002/mbo3.177

来源: Wiley

【 摘 要 】

Abstract

Interspecies communication between Porphyromonas gingivalis and Streptococcus gordonii underlies the development of synergistic dual species communities. Contact with S. gordonii initiates signal transduction within P. gingivalis that is based on protein tyrosine (de)phosphorylation. In this study, we characterize a bacterial tyrosine (BY) kinase (designated Ptk1) of P. gingivalis and demonstrate its involvement in interspecies signaling. Ptk1 can utilize ATP for autophosphorylation and is dephosphorylated by the P. gingivalis tyrosine phosphatase, Ltp1. Community development with S. gordonii is severely abrogated in a ptk1 mutant of P. gingivalis, indicating that tyrosine kinase activity is required for maximal polymicrobial synergy. Ptk1 controls the levels of the transcriptional regulator CdhR and the fimbrial adhesin Mfa1 which mediates binding to S. gordonii. The ptk1 gene is in an operon with two genes involved in exopolysaccharide synthesis, and similar to other BY kinases, Ptk1 is necessary for exopolysaccharide production in P. gingivalis. Ptk1 can phosphorylate the capsule related proteins PGN_0224, a UDP-acetyl-mannosamine dehydrogenase, and PGN_0613, a UDP-glucose dehydrogenase, in P. gingivalis. Knockout of ptk1 in an encapsulated strain of P. gingivalis resulted in loss of capsule production. Collectively these results demonstrate that the P. gingivalis Ptk1 BY kinase regulates interspecies communication and controls heterotypic community development with S. gordonii through adjusting the levels of the Mfa1 adhesin and exopolysaccharide.

【 授权许可】

CC BY   
© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

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