Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome

MicrobiologyOpen

Sagar Chhabra²  Dina Brazil²  John Morrissey¹  James I. Burke³  Fergal O'Gara¹ 

[1] Department of Microbiology, University College Cork, Cork, Ireland;Department of Science and Health, Institute of Technology Carlow, Carlow, Ireland;National Plant Biotechnology Centre, Teagasc OakPark, Carlow, Ireland

关键词: Glucose dehydrogenase;    MPS;    pyrroloquinoline quinone;    soil metagenome.;   

DOI  :  10.1002/mbo3.110

来源: Wiley

【 摘 要 】

Abstract

Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil that had not received P fertilizer over a 15-year period. A fosmid system was used to clone the metagenome of which 18,000 clones (~666 Mb of DNA) was screened for MPS. Functional assays and High Performance Liquid Chromatography analysis recognized gluconic acid production and MPS activity in the range 24.8–77.1 mmol/L and 27.6–38.16 μg/mL, respectively, when screened in an Escherichia coli host (at frequency of one MPS-positive clone hit per 114 Mb DNA tested). The MPS clones (with average insert size of ~37 kb) were analysed by 454 Roche sequencing and annotated. A number of genes/operons with homology to Phosphorous (P) uptake, regulatory and solubilization mechanisms were identified, linking the MPS function to the uncultivated microbiome present in barley rhizosphere soil.

【 授权许可】

CC BY   
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

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