Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents

Microbial Cell Factories

Xiao-Dong Gao¹  Hideki Nakanishi¹  Hongbo Liang²  Yan Xu²  Rongzhen Zhang³  Jingxin Rao⁴ 

[1] 0000 0001 0708 1323, grid.258151.a, Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China;0000 0001 0708 1323, grid.258151.a, Key Laboratory of Industrial Biotechnology of Ministry of Education & School of Biotechnology, Jiangnan University, 214122, Wuxi, People’s Republic of China;0000 0001 0708 1323, grid.258151.a, Key Laboratory of Industrial Biotechnology of Ministry of Education & School of Biotechnology, Jiangnan University, 214122, Wuxi, People’s Republic of China;0000 0001 0708 1323, grid.258151.a, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, People’s Republic of China;0000 0001 2314 964X, grid.41156.37, College of Science of China Pharmaceutical University, 211198, Nanjing, People’s Republic of China;

关键词: S;    Glucose dehydrogenase;    Sustainable enantioselective catalysis;    Organic solvent;    Spore-microencapsulation;   

DOI  :  10.1186/s12934-019-1137-6

来源: publisher

【 摘 要 】

BackgroundSaccharomyces cerevisiae AN120 osw2∆ spores were used as a host with good resistance to unfavorable environment. This work was undertaken to develop a new yeast spore-encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase for efficient chiral synthesis in organic solvents.ResultsThe spore microencapsulation of E228S/SCR II and GDH in S. cerevisiae AN120 osw2∆ catalyzed (R)-phenylethanol in a good yield with an excellent enantioselectivity (up to 99%) within 4 h. It presented good resistance and catalytic functions under extreme temperature and pH conditions. The encapsulation produced several chiral products with over 70% yield and over 99% enantioselectivity in ethyl acetate after being recycled for 4–6 times. It increased substrate concentration over threefold and reduced the reaction time two to threefolds compared to the recombinant Escherichia coli containing E228S and glucose dehydrogenase.ConclusionsThis work first described sustainable enantioselective synthesis without exogenous cofactors in organic solvents using yeast spore-microencapsulation of coupled alcohol dehydrogenases.

【 授权许可】

CC BY