Evaluation of 4 Point‐of‐Care Units for the Determination of Blood l‐Lactate Concentration in Cattle

Journal of Veterinary Internal Medicine

T. Karapinar²  O. Kaynar¹  A. Hayirli³ 

[1] Faculty of Veterinary Medicine, Department of Biochemistry, Ataturk University, Erzurum, Turkey;Faculty of Veterinary Medicine, Department of Internal Medicine, Firat University, Elazig, Turkey;Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Disorders, Ataturk University, Erzurum, Turkey

关键词: Accuracy;    Bovine;    Portable lactate meter;    Precision;    Sensitivity;    Specificity;   

DOI  :  10.1111/jvim.12204

来源: Wiley

【 摘 要 】

Abstract

Background

Despite being used commonly in bovine medicine, information on reliability of point-of-care (POC) lactate meters is limited.

Objective

To determine the validity of 4 commercially available POC lactate meters in cattle.

Animals

Cattle with various diseases (n = 120).

Methods

Blood samples collected from the jugular vein were processed immediately on POC lactate meters. Plasma l-lactate concentration was measured by the enzymatic-colorimetric method (ELISA). Data were subjected to Friedman's test for comparison, Passing-Bablok regression and Bland-Altman plot analyses for reliability, and receiver operating characteristics analysis for sensitivity (Se) and specificity (Sp).

Results

The POC lactate meters were highly correlated with ELISA (r = 0.98–0.99) despite disagreements among units. When regressed on ELISA, blood l-lactate concentrations generated from Accutrend Plus and Lactate Pro were linear up to 16.6 and 15.7 mmol/L, respectively, whereas those generated from i-STAT and Lactate Scout were linear up to 19.5 and 19.7 mmol/L, respectively. All POC lactate meters had a Se of 100% with Sp of 95.7–98.6% at a plasma l-lactate cut-off concentration of 4 mmol/L. i-STAT had the best accuracy (99.0%) and precision (99.8%), the best linear fit (y = −0.13 + 1.04X) yielding the lowest bias (−6.6%) as well as the highest Se (100%) and Sp (98.6%).

Conclusions and Clinical Importance

Despite high correlation with the reference method, dilution is needed for Accutrend Plus/Lactate Pro and i-STAT/Lactate Scout if concentrations >15 and 20 mmol/L, respectively. i-STAT provided the most accurate and precise results.

【 授权许可】

Unknown   
Copyright © 2013 by the American College of Veterinary Internal Medicine