Effect of Heart Failure on Dipeptidyl Peptidase IV Activity in Plasma of Dogs

Journal of Veterinary Internal Medicine

N. Gomez³  V. Matheeussen¹  C. Damoiseaux²  A. Tamborini⁴  A.C. Merveille²  P. Jespers³  C. Michaux⁵  C. Clercx²  I. De Meester¹ 

[1] Laboratory of Medical Biochemistry, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium;Department of Small Animal Clinical Sciences, Faculty of Veterinary Medicine, Université de Liège, Liège, Belgium;Laboratory of Physiology, Faculty of Medicine, Université de Liège, Liège, Belgium;Small Animal Internal Medicine Department, University Veterinary Hospital, University College of Dublin, Dublin, Ireland;Faculty of Medicine, ULB, Brussels, Belgium

关键词: BNP3‐32;    B‐type natriuretic peptide biology;    Dilated cardiomyopathy;    Myxomatous mitral valve disease;   

DOI  :  10.1111/j.1939-1676.2012.00942.x

来源: Wiley

【 摘 要 】

Abstract

Background

In congestive heart failure (HF), plasma B-type natriuretic peptide (BNP) seems devoid of biological effectiveness. BNP_1–32 could be truncated into BNP_3–32 by dipeptidyl peptidase IV (DPP4), and BNP_3–32 has reduced biological activities.

Hypothesis

Increased DPP4 activity is associated with pathophysiology of HF.

Animals

One hundred twenty-eight client-owned dogs and 9 experimental Beagles from the Clinical Veterinary Unit of the University of Liège.

Methods

We prospectively measured plasma DPP4 activity in 5 groups of dogs: normal growing dogs (n = 21), normal adult dogs (n = 60), healthy Beagle (n = 9), dogs with myxomatous mitral valve disease (n = 35), and dogs with dilated cardiomyopathy (n = 12). The final diagnosis and the severity of HF were determined by Doppler echocardiography. Plasma DPP4 activity was measured kinetically by a fluorimetric method.

Results

In growing dogs, DPP4 activity was higher than in adults (P < .001) and inversely correlated with age (r = −0.57, P < .01). In adults, DPP4 activity increased linearly with body weight (r = 0.39, P < .01), but there was no influence of age or sex. No effect of the circadian rhythm was noted. DPP4 activity was significantly higher in HF ISACHC I (16.3 ± 1.14 U/L) compared with healthy adults (12.4 ± 0.65 U/L, P < .05) and HF ISACHC III (11.0 ± 1.50 U/L, P < .05). Mean DPP4 activity in ISACHC II was 15.1 ± 1.4 U/L.

Conclusion and Clinical Importance

We did not find evidence that plasma DPP4 activity is responsible for the “BNP resistance” in overt congestive HF, but it may be implicated in early stages.

【 授权许可】

Unknown   
Copyright © 2012 by the American College of Veterinary Internal Medicine