Resting Energy Expenditure per Lean Body Mass Determined by Indirect Calorimetry and Bioelectrical Impedance Analysis in Cats

Journal of Veterinary Internal Medicine

S.A. Center²  K.L. Warner¹  J.F. Randolph¹  J.J. Wakshlag¹ 

[1] Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY;Pet Care Research and Development, Iams Co, Proctor & Gamble, Lewisburg, OH

关键词: Body condition score;    Deuterium;    Doubly labeled water;    Feline;   

DOI  :  10.1111/j.1939-1676.2011.00824.x

来源: Wiley

【 摘 要 】

Abstract

Background

Resting energy expenditure (REE) approximates ≥60% of daily energy expenditure (DEE). Accurate REE determination could facilitate sequential comparisons among patients and diseases if normalized against lean body mass (LBM).

Objective

(1) Validate open-flow indirect calorimetry (IC) system and multifrequency bioelectrical impedance analysis (MF-BIA) to determine REE and LBM, respectively, in healthy nonsedated cats of varied body conditions; (2) normalize REE against LBM.

Animals

Fifty-seven adult neutered domestic short-haired cats with stable BW.

Methods

Continuous (45-min) IC-measurements determined least observed metabolism REE. Cage gas flow regulated with mass flow controllers was verified using nitrogen dilution; span gases calibrated gas measurements. Respiratory quotient accuracy was verified using alcohol combustion. IC-REE was compared to DEE, determined using doubly labeled water. MF-BIA LBM was validated against criterion references (deuterium, sodium bromide). Intra- and interassay variation was determined for IC and MF-BIA.

Results

Mean IC-REE (175 ± 38.7 kcal; 1.5–14% intra- and interassay CV%) represented 61 ± 14.3% of DEE. Best MF-BIA measurements were collected in sternal recumbency and with electrodes in neck-tail configuration. MF-BIA LBM was not significantly different from criterion references and generated LBM interassay CV% of 6.6–10.1%. Over- and underconditioned cats had significantly (P ≤ .05) lower and higher IC-REE (kcal/kg) respectively, compared with normal-conditioned cats. However, differences resolved with REE/LBM (approximating 53 ± 10.3 kcal/LBM [kg]).

Conclusions and Clinical Importance

IC and MF-BIA validated herein reasonably estimate REE and LBM in cats. REE/LBM(kg) may permit comparison of energy utilization in sequential studies or among different cats.

【 授权许可】

Unknown   
Copyright © 2011 by the American College of Veterinary Internal Medicine