The role of pleural fluid MAGE RT‐nested PCR in the diagnosis of malignant pleural effusion

Thoracic Cancer

Eun Ju Jeon²  Hye Kyeong Park²  Kyeongman Jeon²  Won-Jung Koh²  Gee Young Suh²  Man Pyo Chung²  Hojoong Kim²  O. Jung Kwon²  Chang-Seok Ki¹  Jong-Won Kim¹  Young Mog Shim³ 

[1] Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea;Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea;Department of Thoracic and Cardiovascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

关键词: carcinoembryonic antigen;    cytology;    malignancy;    melanoma antigen (MAGE);    pleural effusion;   

DOI  :  10.1111/j.1759-7714.2012.00131.x

来源: Wiley

【 摘 要 】

Abstract

Background:  Melanoma antigen (MAGE) genes are expressed in tumor cells, the testis and the placenta. The purpose of this prospective study was to investigate the sensitivity, specificity, and accuracy of the carcinoembryonic antigen (CEA), MAGE reverse transcriptase-nested polymerase chain reaction (RT-nested PCR), and cytology of pleural fluid in the diagnosis of malignant pleural effusion.

Methods:  Patients in whom unilateral pleural effusion was identified on chest radiography from January to December 2009 were included in the study. MAGE genes were analyzed by RT-nested PCR using MAGE A1-6 common primers.

Results:  Of 81 enrolled patients, 46 were diagnosed as malignant pleural effusion, and 24 were diagnosed as benign pleural effusion. The diagnoses of 11 patients were not confirmed in this study. The diagnostic sensitivity, specificity, and accuracy of MAGE RT-nested PCR were 61.4%, 95.7%, and 73.1%, respectively. The diagnostic sensitivities of cytology and CEA (>5 ng/mL) were 61.4% and 75.0%, respectively. Among 17 patients with negative cytology who had malignant pleural effusion, 12 and 10 patients were positive for CEA (>5.0 ng/mL) and MAGE RT-nested PCR, respectively. However, of five patients with malignant pleural effusion that was not recognized by cytology and CEA, MAGE RT-nested PCR correctly predicted a malignant etiology in only one additional patient (20%).

Conclusions:  MAGE RT-nested PCR seems to add little on the combination of conventional methods in the diagnosis of malignant effusion.

【 授权许可】

Unknown   
© 2012 Tianjin Lung Cancer Institute and Wiley Publishing Asia Pty. Ltd