Low glucose microenvironment of normal kidney cells stabilizes a subset of messengers involved in angiogenesis

Physiological Reports

Elodie de Laplanche²  Asma Boudria³  Estelle Dacheux²  Anne Vincent²  Nicolas Gadot²  Fouzia Assade²  Katy Le Corf²  Xavier Leroy⁴  Florence Mège Lechevallier¹  Béatrice Eymin³  Nicole Dalla Venezia² 

[1] Department of Pathology, Hôpital Edouard Herriot, Lyon, France;Université de Lyon, Lyon, France;Institut Albert Bonniot Equipe 2 Bases Moléculaires de la Progression des Cancers du Poumon, INSERM U823/Université Joseph Fourier, Grenoble, France;Institut de Pathologie, CHRU, Faculté de Médecine, Université de Lille, Lille, France

关键词: Glucose sensing;    HIF1A;    metabolism;    normal renal tubule;    VEGFA;   

DOI  :  10.14814/phy2.12253

来源: Wiley

【 摘 要 】

Abstract

As glucose is a mandatory nutrient for cell proliferation and renewal, it is suspected that glucose microenvironment is sensed by all cell types to regulate angiogenesis. Several glucose-sensing components have been partially described to respond to high glucose levels. However, little is known about the response to low glucose. Here, we used well-differentiated isolated normal rat renal tubules under normal oxygenation conditions to assess the angiogenic response to low glucose. In apparent paradox, but confirming observations made separately in other models, high glucose but also low glucose increased mRNA level of vascular endothelial growth factor A (VEGFA). A subset of mRNAs including hypoxia-inducible factor 1A (HIF1A), angiopoietin receptor (TIE-2), and VEGF receptor 2 (FLK1) were similarly glucose-sensitive and responded to low glucose by increased stability independently of HIF1A and HIF2A proteins. These results contribute to gain some insights as to how normal cells response to low glucose may play a role in the tumor microenvironment.

【 授权许可】

CC BY   
© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

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