The rat adequately reflects human responses to exercise in blood biochemical profile: a comparative study

Physiological Reports

Georgios Goutianos¹  Aikaterini Tzioura¹  Antonios Kyparos¹  Vassilis Paschalis²  Nikos V. Margaritelis¹  Aristidis S. Veskoukis¹  Andreas Zafeiridis¹  Konstantina Dipla¹  Michalis G. Nikolaidis¹ 

[1] Exercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Serres, Greece;Department of Physical Education and Sport Science, University of Thessaly, Karies, Trikala, Greece

关键词: Animal models;    biomarkers;    blood;    exercise;    human;    rat;   

DOI  :  10.14814/phy2.12293

来源: Wiley

【 摘 要 】

Abstract

Animal models are widely used in biology and the findings of animal research are traditionally projected to humans. However, recent publications have raised concerns with regard to what extent animals and humans respond similar to physiological stimuli. Original data on direct in vivo comparison between animals and humans are scarce and no study has addressed this issue after exercise. We aimed to compare side by side in the same experimental setup rat and human responses to an acute exercise bout of matched intensity and duration. Rats and humans ran on a treadmill at 86% of maximal velocity until exhaustion. Pre and post exercise we measured 30 blood chemistry parameters, which evaluate iron status, lipid profile, glucose regulation, protein metabolism, liver, and renal function. ANOVA indicated that almost all biochemical parameters followed a similar alteration pattern post exercise in rats and humans. In fact, there were only 2/30 significant species × exercise interactions (in testosterone and globulins), indicating different responses to exercise between rats and humans. On the contrary, the main effect of exercise was significant in 15/30 parameters and marginally nonsignificant in other two parameters (copper, P = 0.060 and apolipoprotein B, P = 0.058). Our major finding is that the rat adequately mimics human responses to exercise in those basic blood biochemical parameters reported here. The physiological resemblance of rat and human blood responses after exercise to exhaustion on a treadmill indicates that the use of blood chemistry in rats for exercise physiology research is justified.

【 授权许可】

CC BY   
© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

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