Intramuscular anabolic signaling and endocrine response following high volume and high intensity resistance exercise protocols in trained men

Physiological Reports

Adam M. Gonzalez¹  Jay R. Hoffman¹  Jeremy R. Townsend¹  Adam R. Jajtner¹  Carleigh H. Boone¹  Kyle S. Beyer¹  Kayla M. Baker¹  Adam J. Wells¹  Gerald T. Mangine¹  Edward H. Robinson IV¹  David D. Church¹  Leonardo P. Oliveira¹  Darryn S. Willoughby²  David H. Fukuda¹ 

[1] Institute of Exercise Physiology and Wellness, Sport and Exercise Science, University of Central Florida, Orlando, Florida, USA;Exercise and Biochemical Nutrition Laboratory, Baylor University, Waco, Texas, USA

关键词: Hormone hypothesis;    intensity;    mTOR pathway;    mTORC1;    volume;   

DOI  :  10.14814/phy2.12466

来源: Wiley

【 摘 要 】

Abstract

Resistance exercise paradigms are often divided into high volume (HV) or high intensity (HI) protocols, however, it is unknown whether these protocols differentially stimulate mTORC1 signaling. The purpose of this study was to examine mTORC1 signaling in conjunction with circulating hormone concentrations following a typical HV and HI lower-body resistance exercise protocol. Ten resistance-trained men (24.7 ± 3.4 years; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) performed each resistance exercise protocol in a random, counterbalanced order. Blood samples were obtained at baseline (BL), immediately (IP), 30 min (30P), 1 h (1H), 2 h (2H), and 5 h (5H) postexercise. Fine needle muscle biopsies were completed at BL, 1H, and 5H. Electromyography of the vastus lateralis was also recorded during each protocol. HV and HI produced a similar magnitude of muscle activation across sets. Myoglobin and lactate dehydrogenase concentrations were significantly greater following HI compared to HV (P = 0.01–0.02), whereas the lactate response was significantly higher following HV compared to HI (P = 0.003). The growth hormone, cortisol, and insulin responses were significantly greater following HV compared to HI (P = 0.0001–0.04). No significant differences between protocols were observed for the IGF-1 or testosterone response. Intramuscular anabolic signaling analysis revealed a significantly greater (P = 0.03) phosphorylation of IGF-1 receptor at 1H following HV compared to HI. Phosphorylation status of all other signaling proteins including mTOR, p70S6k, and RPS6 were not significantly different between trials. Despite significant differences in markers of muscle damage and the endocrine response following HV and HI, both protocols appeared to elicit similar mTORC1 activation in resistance-trained men.

【 授权许可】

CC BY   
© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

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