Iron handling in hippocampal neurons: activity‐dependent iron entry and mitochondria‐mediated neurotoxicity

Aging Cell

Ilaria Pelizzoni¹  Romina Macco¹  Marco Francesco Morini¹  Daniele Zacchetti¹  Fabio Grohovaz¹ 

[1] Cellular Neurophysiology Unit, Division of Neuroscience, San Raffaele Scientific Institute, via Olgettina 58, I-20132 Milano, Italy

关键词: calcium;    hippocampal neurons;    iron;    mitochondria;    neurotoxicity;    oxidative stress;   

DOI  :  10.1111/j.1474-9726.2010.00652.x

来源: Wiley

【 摘 要 】

Summary

The characterization of iron handling in neurons is still lacking, with contradictory and incomplete results. In particular, the relevance of non-transferrin-bound iron (NTBI), under physiologic conditions, during aging and in neurodegenerative disorders, is undetermined. This study investigates the mechanisms underlying NTBI entry into primary hippocampal neurons and evaluates the consequence of iron elevation on neuronal viability. Fluorescence-based single cell analysis revealed that an increase in extracellular free Fe²⁺ (the main component of NTBI pool) is sufficient to promote Fe²⁺ entry and that activation of either N-methyl-d-aspartate receptors (NMDARs) or voltage operated calcium channels (VOCCs) significantly potentiates this pathway, independently of changes in intracellular Ca²⁺ concentration ([Ca²⁺]_i). The enhancement of Fe²⁺ influx was accompanied by a corresponding elevation of reactive oxygen species (ROS) production and higher susceptibility of neurons to death. Interestingly, iron vulnerability increased in aged cultures. Scavenging of mitochondrial ROS was the most powerful protective treatment against iron overload, being able to preserve the mitochondrial membrane potential and to safeguard the morphologic integrity of these organelles. Overall, we demonstrate for the first time that Fe²⁺ and Ca²⁺ compete for common routes (i.e. NMDARs and different types of VOCCs) to enter primary neurons. These iron entry pathways are not controlled by the intracellular iron level and can be harmful for neurons during aging and in conditions of elevated NTBI levels. Finally, our data draw the attention to mitochondria as a potential target for the treatment of the neurodegenerative processes induced by iron dysmetabolism.

【 授权许可】

Unknown   
© 2011 The Authors. Aging Cell © 2011 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland