Selenoprotein W expression and regulation in mouse brain and neurons

Brain and Behavior

Arjun V. Raman¹  Matthew W. Pitts¹  Ali Seyedali¹  Ann C. Hashimoto¹  Frederick P. Bellinger¹ 

[1] Cell and Molecular Biology Department, John A. Burns School of Medicine, University of Hawai'i at Manoa, Honolulu, Hawaii

关键词: Selenium;    selenocysteine;    selenoprotein;    selenoprotein P;    selenoprotein W;   

DOI  :  10.1002/brb3.159

来源: Wiley

【 摘 要 】

Abstract

Background

Selenoprotein W (Sepw1) is a selenium-containing protein that is abundant in brain and muscle of vertebrate animals. Muscular expression of Sepw1 is reduced by dietary selenium (Se) deficiency in mammals, whereas brain expression is maintained. However, expression of Sepw1 depends on the Se transporter selenoprotein P (Sepp1).

Methods

We assessed the regional and cellular expression of Sepw1 in the mouse brain and neuronal cultures.

Results

We found that Sepw1 is widespread in neurons and neuropil of mouse brain and appears in both the soma and processes of neurons in culture. Pyramidal neurons of cortex and hippocampus express high levels of Sepw1. It is also abundant in Purkinje neurons and their dendritic arbors in the cerebellum. Analysis of synaptosome fractions prepared from mice brains indicated that Sepw1 is present at synapses, as were several proteins involved in selenoprotein synthesis. Synaptic expression of Sepw1 expression is reduced in mice lacking Sepp1 compared with control mice, although selenoprotein synthesis factors were similarly expressed in both genotypes. Lastly, Sepw1 mRNA coimmunoprecipitates with Staufen 2 protein in a human neuronal cell line.

Conclusions

Our results suggest that Sepw1 may be locally synthesized in distal compartments of neurons including synapses.

【 授权许可】

CC BY   
© 2013 The Authors. Brain and Behavior published by Wiley Periodicals, Inc.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.