Caloric restriction reveals a metabolomic and lipidomic signature in liver of male mice

Aging Cell

Mariona Jové¹  Alba Naudí¹  Omar Ramírez-Núñez¹  Manuel Portero-Otín¹  Colin Selman²  Dominic J. Withers³ 

[1] Department of Experimental Medicine, University of Lleida-Biomedical Research Institute of Lleida, Lleida, Spain;Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow, UK;Metabolic Signaling Group, Medical Research Council Clinical Sciences Centre, Imperial College London, London, UK

关键词: caloric restriction;    glycolytic pathway;    phospholipids oxidation;    protein oxidative damage;    tryptophan pathway;   

DOI  :  10.1111/acel.12241

来源: Wiley

【 摘 要 】

Summary

Lipid composition, particularly membrane unsaturation, has been proposed as being a lifespan determinant, but it is currently unknown whether caloric restriction (CR), an accepted life-extending intervention, affects cellular lipid profiles. In this study, we employ a liquid chromatography quadrupole time-of-flight-based methodology to demonstrate that CR in the liver of male C57BL/6 mice: (i) induces marked changes in the cellular lipidome, (ii) specifically reduces levels of a phospholipid peroxidation product, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphatidylcholine, (iii) alters cellular phosphoethanolamine and triglyceride distributional profiles, (iv) affects mitochondrial electron transport chain complexes, increasing complex II and decreasing complex III and (v) is associated with specific changes in liver metabolic pathways. These data demonstrate that CR induces a specific lipidome and metabolome reprogramming event in mouse liver which is associated with lower protein oxidative damage, as assessed by mass spectrometry-based measurements. Such changes may be critical to the increased lifespan and healthspan observed in C57BL/6 mice following CR.

【 授权许可】

CC BY   
© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.