Homeostatic and injury‐induced microglia behavior in the aging brain

Aging Cell

Jasmin K. Hefendehl¹  Jonas J. Neher¹  Rafael B. Sühs¹  Shinichi Kohsaka²  Angelos Skodras¹ 

[1] Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany;Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan

关键词: aging;    in vivo imaging;    microglia;    neocortex;    neurodegeneration;   

DOI  :  10.1111/acel.12149

来源: Wiley

【 摘 要 】

Summary

Microglia cells are essential for brain homeostasis and have essential roles in neurodegenerative diseases. Aging is the main risk factor for most neurodegenerative diseases, and age-related changes in microglia may contribute to the susceptibility of the aging brain to dysfunction and neurodegeneration. We have analyzed morphology and dynamic behavior of neocortical microglia in their physiological environment in young adult (3-month-old), adult (11- to 12-month-old), and aged (26- to 27-month-old) C57BL/6J-Iba1-eGFP mice using in vivo 2-photon microscopy. Results show that surveying microglial cells in the neocortex exhibit age-related soma volume increase, shortening of processes, and loss of homogeneous tissue distribution. Furthermore, microglial process speed significantly decreased with age. While only a small population of microglia showed soma movement in adult mice, the microglia population with soma movement was increased in aged mice. However, in response to tissue injury, the dynamic microglial response was age-dependently diminished. These results provide novel insights into microglial behavior and indicate that microglial dysfunction in the aging brain may contribute to age-related cognitive decline and neurodegenerative diseases.

【 授权许可】

Unknown   
© 2013 the Anatomical Society and John Wiley & Sons Ltd