Next-Generation Sequencing Reveals the Progression of COVID-19

Frontiers in Cellular and Infection Microbiology

Xiaokun Li¹  Shengwei Jin²  Jinyu Wu³  Xin Xu³  Kun Pang³  Xiaomin Chen³  Jing Luo⁴  Yutong Kang⁵ 

[1] Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China;Department of Anesthesia and Critical Care, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China;Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou, China;Rheumatology Department, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China;Wenzhou Key Laboratory of Sanitary Microbiology, Ministry of Education, Wenzhou, China;Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou, China;School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China;

关键词: next-generation sequencing;    SARS coronavirus 2 (SARS-CoV-2);    origin;    gut microbiota;    angiotensin-converting enzyme 2 (ACE2);   

DOI  :  10.3389/fcimb.2021.632490

来源: Frontiers

【 摘 要 】

The novel coronavirus SARS-CoV-2 (causing the disease COVID-19) has caused a highly transmissible and ongoing pandemic worldwide. Due to its rapid development, next-generation sequencing plays vital roles in many aspects. Here, we summarize the current knowledge on the origin and human transmission of SARS-CoV-2 based on NGS analysis. The ACE2 expression levels in various human tissues and relevant cells were compared to provide insights into the mechanism of SAS-CoV-2 infection. Gut microbiota dysbiosis observed by metagenome sequencing and the immunogenetics of COVID-19 patients according to single-cell sequencing analysis were also highlighted. Overall, the application of these sequencing techniques could be meaningful for finding novel intermediate SARS-CoV-2 hosts to block interspecies transmission. This information will further benefit SARS-CoV-2 diagnostic development and new therapeutic target discovery. The extensive application of NGS will provide powerful support for our fight against future public health emergencies.

【 授权许可】

CC BY