The Anti-Inflammatory Effect and Mucosal Barrier Protection of Clostridium butyricum RH2 in Ceftriaxone-Induced Intestinal Dysbacteriosis

Frontiers in Cellular and Infection Microbiology

Xue Sui¹  Yuyuan Li¹  Xiaoqing Wei²  Chunzheng Liu²  He Liu²  Qiao Lu²  Ming Li²  Yiqin Cheng²  Xin Wang²  Yinhui Liu²  Jieli Yuan²  Weikang Ye²  Binbin Gao²  Man Liu³  Hao Cheng⁴  Lu Zhang⁴ 

[1] Advanced Institute for Medical Sciences, Dalian Medical University, Dalian, China;College of Basic Medical Science, Dalian Medical University, Dalian, China;College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China;Marketing Department, Hangzhou Grand Biologic Pharmaceutical Inc., Hangzhou, China;

关键词: Clostridium butyricum;    CB RH2 in Intestinal Dysbiosis;    immune response;    mucosal barrier function;    gut microbiota;   

DOI  :  10.3389/fcimb.2021.647048

来源: Frontiers

【 摘 要 】

This study aimed at determining the beneficial effect of Clostridium butyricum (CB) RH2 on ceftriaxone-induced dysbacteriosis. To this purpose, BALB/c mice were exposed to ceftriaxone (400 mg/ml) or not (control) for 7 days, and administered a daily oral gavage of low-, and high-dose CB RH2 (108 and 1010 CFU/ml, respectively) for 2 weeks. CB RH2 altered the diversity of gut microbiota, changed the composition of gut microbiota in phylum and genus level, decreased the F/B ratio, and decreased the pro-inflammatory bacteria (Deferribacteres, Oscillibacter, Desulfovibrio, Mucispirillum and Parabacteroides) in ceftriaxone-treated mice. Additionally, CB RH2 improved colonic architecture and intestinal integrity by improving the mucous layer and the tight junction barrier. Furthermore, CB RH2 also mitigated intestinal inflammation through decreasing proinflammatory factors (TNF-α and COX-2) and increasing anti-inflammatory factors (IL-10). CB RH2 had direct effects on the expansion of CD4+ T cells in Peyer’s patches (PPs) in vitro, which in turn affected their immune response upon challenge with ceftriaxone. All these data suggested that CB RH2 possessed the ability to modulate the intestinal mucosal and systemic immune system in limiting intestinal alterations to relieve ceftriaxone-induced dysbacteriosis.

【 授权许可】

CC BY