Distribution and Variation of Serotypes and Pneumococcal Surface Protein A Clades of Streptococcus pneumoniae Strains Isolated From Adult Patients With Invasive Pneumococcal Disease in Japan

Frontiers in Cellular and Infection Microbiology

Kei Kasahara¹  Makoto Ohnishi²  Bin Chang²  Masatomo Morita²  Yuki Kinjo³  Kengo Oshima⁴  Hiroshi Watanabe⁵  Jiro Fujita⁶  Takaya Maruyama⁷  Junichiro Nishi⁸  Tetsuya Kubota⁹  Koji Kuronuma^1,10  Yoshinari Tanabe^1,11  Shigeru Suga^1,12  Kazunori Oishi^1,13  Kosuke Tamura^1,13  Shuichi Abe^1,14 

[1] Center for Infectious Diseases, Nara Medical University, Nara, Japan;Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan;Department of Bacteriology, The Jikei University School of Medicine, Tokyo, Japan;Jikei Center for Biofilm Science and Technology, The Jikei University School of Medicine, Tokyo, Japan;Department of Intelligent Network for Infection Control, Tohoku University Graduate School of Medicine, Sendai, Japan;Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Miyagi, Japan;Department of Infection Control and Prevention, Kurume University School of Medicine, Fukuoka, Japan;Department of Infectious, Respiratory, and Digestive Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan;Department of Medicine, National Hospital Organization, Mie Hospital, Mie, Japan;Department of Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan;Department of Respiratory Medicine and Allergology, Kochi Medical School, Kochi University, Kochi, Japan;Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, Hokkaido, Japan;Department of Respiratory Medicine, Niigata Prefectural Shibata Hospital, Niigata, Japan;National Hospital Organization Mie National Hospital, Mie, Japan;Toyama Institute of Health, Toyama, Japan;Yamagata Prefectural Central Hospital, Yamagata, Japan;

关键词: Streptococcus pneumoniae;    invasive pneumococcal disease;    serotype;    pneumococcal surface protein A clade;    vaccine;    Japan;    adults;   

DOI  :  10.3389/fcimb.2021.617573

来源: Frontiers

【 摘 要 】

Pneumococcal surface protein A (PspA) is a surface protein of Streptococcus pneumoniae that may be a candidate antigen for new pneumococcal vaccines. This study investigates the distribution of PspA clades of the causative strains of adult invasive pneumococcal disease (IPD) in Japan. Of the 1,939 strains isolated from cases of adult IPD during 2014–2019, the PspA clades of 1,932 (99.6%) strains were determined, and no pspA was detected in the remaining 7 strains (0.4%). PspA clades 1–6 were detected in 786 (40.5%), 291 (15.0%), 443 (22.8%), 369 (19.0%), 33 (1.7%), and 6 (0.3%) strains, respectively. New PspA clades (0.2%) were identified in two non-typeable and two serotype 35B pneumococci. The proportions of clade 1 and clade 2 showed significantly decreased and increased trends, respectively. Furthermore, the PspA clade of pneumococcal strains was partially serotype- and sequence type-dependent. The majority of strains belonging to serotypes contained in both the 13-valent pneumococcal conjugate vaccine (PCV13) and the 23-valent pneumococcal polysaccharide vaccine (PPSV23) belonged to PspA clades 1 or 3. In contrast, the distribution of clades in non-vaccine serotypes was wider than that of vaccine serotype pneumococci. Our findings demonstrate that almost all pneumococcal strains from adult IPD express PspA clades 1–4, especially for non-vaccine serotypes. These results may be useful for the development of a new pneumococcal vaccine with PspA.

【 授权许可】

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