AC-electric-field-induced parthenogenesis of mouse oocyte

Micro & nano letters

article

Hong-Yuan Huang¹  Wei-Lun Kao²  Yi-Wen Wang¹  Da-Jeng Yao² 

[1] Department of Obstetrics and Gynecology, Lin-Kou Medical Center, Chang Gung Memorial Hospital;Department of Power Mechanical Engineering, National Tsing Hua University;Institute of NanoEngineering and MicroSystems, National Tsing Hua University

关键词: biological effects of fields;    bioelectric phenomena;    cellular effects of radiation;    microfluidics;    AC-electric-field-induced parthenogenesis;    mouse oocyte;    microfluidic chip;    sperms;    sperm insemination;    electrodes;    glass chip;    ITO wet etching;    electric field;    trapping durations;    statistical difference;    trapping time;    AC p-DEP electric activation;    control group;    artificial reproduction;   

DOI  :  10.1049/mnl.2017.0354

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

The aldose reductase pathway proves that elevated blood glucose promotes cellular dysfunction. The polyol pathway converts excess intracellular glucose into alcohols via activity of the aldose reductase. This enzyme catalyzes the conversion of glucose to sorbitol which triggers variety of intracellular changes in the tissues. Among diabetes, activity is drastically increased in association with three main consequences inside the cells. The aim of this study was to detect the association of the C-106 T polymorphism of the aldose reductase gene and its frequency among a sample of 150 Egyptian adults with type 2 diabetic patients having diabetic microvascular. The detection of the aldose reductase C-106 T polymorphism gene was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype distribution of the C-106 T polymorphism showed that CC genotype was statistically significantly higher among patients with retinopathy compared to nephropathy. Patients with nephropathy had significant association with the TT genotype when compared with diabetic retinopathy patients. Follow up study after the genotype detection among recently diagnosed diabetic patients in order to give a prophylactic aldose reductase inhibitors; studying the microvascular complications and its relation to the genotype polymorphisms. The study may include multiple gene polymorphisms to make the relation between the gene and the occurrence of these complications more evident.

【 授权许可】

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