Anticancer activity evaluation of green synthesised gold–silver alloy nanoparticles on colourectal HT-29 and prostate DU-145 carcinoma cell lines

Micro & nano letters

article

Reza Entezari Heravi¹  Sajad Zakeri²  Pardis Nazari¹ 

[1]Pharmaceutical Biotechnology Department, Faculty of Pharmacy, Zabol University of Medical Sciences

[2]Student Research Committee, Zabol University of Medical Sciences

关键词: patient treatment;    nanomedicine;    biomedical materials;    cancer;    drug delivery systems;    nanobiotechnology;    tumours;    nanoparticles;    toxicology;    drugs;    anticancer activity evaluation;    green synthesised gold–silver alloy nanoparticles;    colorectal HT-29;    DU-145 carcinoma cell lines;    metal nanoparticles;    anticancer properties;    green method;    antitumour activity;    biosynthesised nanoparticles;    colon cell lines;    cancer cell lines;    viable cell count;    spherical Au–Ag alloy nanoparticles;    growth inhibition;    human prostate;    DU-145 cell line;    HT-29 cell line;    active substance;    tumour cell lines;    Au–Ag alloy nanoparticle activity;   

DOI  :  10.1049/mnl.2018.0235

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

The plant Abrus precatorius, which belong to Leguminosae (Fabaceae) family and known as Crab’s eyes, Rosary pea with characteristic red and black seeds. It was used in folk medicine in India, China and East Asian countries for treatment of various diseases.    The plant was extracted by '' general method of extraction'' (Harborne, 1973) using 80% aqueous ethanol as a solvent of extraction by soxhlet apparatus. Preliminary qualitative phytochemical screening were performed on the crude ethanolic extract  and revealed the presence of alkaloids, flavonoids ,terpenoids and phytosterols in Iraqi Abrus precatorius plant. Three different fractions were obtained from crude extract which are fraction one (chloroform fraction), fraction two (ethyl acetate fraction), and fraction three (petroleum ether fraction) which are represent alkaloids, flavonoids and steroids respectively. The alkaloid abrine was isolated from the chloroform fraction  in pure form by using preparative thin layer chromatography (PTLC) and then subjected to different physico-chemical and specteral analytical techniques to identify its chemical structure: melting point (M.P.), thin layer chromatography (TLC), high performance liquid chromatography (HPLC) , fourier transforms infrared spectra (FT-IR) and elemental microanalysis (CHNO).

【 授权许可】

CC BY|CC BY-ND|CC BY-NC|CC BY-NC-ND