Fast immunofluorescence lateral flow test strip approach for detection of homocysteine

Micro & nano letters

article

Wei Chen¹  Chaoman Ang¹  Doudou Lou¹  Yongxin Ji²  Ling Huang²  Yefei Zhu³  Zhirui Guo³  Ning Gu¹  Yu Zhang¹ 

[1] State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering and Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University;Ltd;Laboratory Medicine Center, The Second Affiliated Hospital of Nanjing Medical University

关键词: nanomedicine;    fluorescence;    diseases;    enzymes;    catalysis;    polymer blends;    biosensors;    molecular biophysics;    nanofabrication;    cardiovascular system;    bio-optics;    fast immunofluorescence lateral flow test strip;    SAH-bovine serum albumin;    Hcy S-methyltransferase catalysis;    S-adenosyl methionine;    S-adenosyl Hcy;    biotin-antibody;    signal amplification;    streptavidin;    fluorophores;    homocysteine detection;    fluorescence intensity;    conjugate pad;    sample diluents;    signal amplification label;    poly(styrene-acrylate) copolymer nanospheres;    fluorescent nanospheres;    cardiovascular disease;    independent risk factor;   

DOI  :  10.1049/mnl.2018.5040

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Cisplatin (CP), a platinum compound, is one of the most active cytotoxic drugs used for cancer treatment. Nephrotoxicity is severe dose limiting side effect of this drug. Abnormal production of reactive oxygen species (ROSs) leading to oxidative stress has been implicated in kidney toxicity by Cisplatin. Here the study was aimed to evaluate nephroprotective effect of ethanolic extract of Terminalia arjuna bark (EETAB) at the doses (200 & 400 mg/kg, body weight) against Cisplatin (7.5 mg/kg, i.p) induced nephrotoxicity in rats. The evaluation was done by measuring % change in body weight, renal function tests such as Blood Urea Nitrogen (BUN), Serum Creatinine (Cr), Serum Total Protein (TP) and also Kidney SOD (Superoxide dismutase),CAT (Catalase), GSH (Reduced glutathione) and MDA (Malondialdehyde) levels altered by Cisplatin administration. Rats treated with EETAB2 (400mg/kg) significantly (P<0.001) reduced the elevated levels of BUN, Cr, TP, MDA and significantly (P<0.001) increased the levels of SOD, GSH, and CAT by restoring kidney architecture. In conclusion EETAB2 (400mg/kg) attractively showed the protection against Cisplatin nephrotoxicity.

【 授权许可】

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