Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide

Micro & nano letters

article

Wenjun Li¹  Tingting Jiang²  Yang Pu³  Xudong Jiao¹  Wenqiang Tan¹  Song Qin¹ 

[1] Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences;School of Life Science, Ludong University;School of Agriculture, Ludong University

关键词: biochemistry;    biosensors;    nanosensors;    nanofabrication;    fluorescence;    molecular biophysics;    radiation quenching;    chemical sensors;    sugar;    proteins;    biomedical optical imaging;    graphene compounds;    GO-CS;    low concentrations;    competitive binding;    maltose-binding protein;    recombinant phycocyanin;    far-red fluorescence;    MBP-rPC;    rPC emission;    rPC fluorescence;    linear detection range;    glucose biosensor;    fluorescence quenching;    chitosan-modified graphene oxide;    low-molecular-weight chitosan;    glucose detection;    fluorescence resonance energy transfer;    graphene oxide nanomaterial;    mass 0.1 mg to 1.0 mg;    CO;   

DOI  :  10.1049/mnl.2018.5269

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Metoclopramide (MCP) ion selective electrodes based on metoclopramide-phosphotungstic acid (MCP-PT) ion pair complex in PVC matrix membrane were constructed. The plasticizers used were tri-butyl phosphate (TBP), di-octyl phenyl phosphonate (DOPP), di-butyl phthalate (DBPH), di-octyl phthalate (DOP), di-butyl phosphate (DBP), bis 2-ethyl hexyl phosphate (BEHP). The sensors based on TBP, DOPP, DBPH and DOP display a fast, stable and linear response with slopes 59.9, 57.7, 57.4, 55.3 mV/decade respectively at pH ranged 2-6. The linear concentration range between 1.0×10-5 – 1.0×10-2 M with detection limit 3.0×10-6 and 4.0×10-6 M for electrodes using TBP, DOPP and DBPH while electrode using DOP shows a linear concentration range between 3.0×10-5 – 1.0×10-2 M and detection limit 6×10-6 M. The selectivity coefficient values were calculated for MCP with respect to different inorganic cations, sugars and amino acids using the MCP depending on TBP plasticizer which is also used for the determination of MCP in pure solutions and in pharmaceutical preparations by direct, standard addition methods and as an indicator electrode in potentiometric titration with phosphotungstic acid (PT) giving satisfactory results.

【 授权许可】

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