Application of β -CD/HA composite as a potential SD carrier to improve the dissolution of curcumin

Micro & nano letters

article

Defeng Xu¹  Wenjie Wang¹  Mengmeng Yang¹  Sarit B. Bhaduri²  Chingcheng Huang³  Lei Yang⁵  Zhenyi Zhang⁶  Huan Zhou³ 

[1] School of Pharmaceutical Engineering and Life Science, Changzhou University;Department of Mechanical, Industrial and Manufacturing Engineering, University of Toledo;School of Mechanical Engineering, Jiangsu University of Technology;Department of Biomedical Engineering, Mingchuan University;Department of Orthopaedics, Orthopaedic Institute, the First Affiliated Hospital, Soochow University;Center for Material Cycles and Waste Management Research, National Institute for Environmental Studies;Division of Environmental Technology, Institute of Nuclear and New Energy Technology, Tsinghua University

关键词: chromatography;    drugs;    dissolving;    transmission electron microscopy;    biomedical materials;    scanning electron microscopy;    drug delivery systems;    X-ray diffraction;    particle size;    Fourier transform infrared spectra;    materials preparation;    composite materials;    dissolution rate;    released curcumin;    potential SD carrier;    solid dispersion formulation;    cyclodextrin;    curcumin release;    CD-HA composite;    β-CD-HA–curcumin;    antioxidant activity;    curcumin dissolution;    X-ray diffraction;    transmission electron microscopy;    scanning electron microscopy;    pore size analysis;    Fourier transform infrared spectroscopy;    specific surface area;    high-performance liquid chromatography;   

DOI  :  10.1049/mnl.2018.5096

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

The aim of this study is to evaluate in-vitro activity of Cefamandol (Cfm) and Ceftazidime (Cfz), in combination with Clavulanic acid (CA) against ten complicated multiresistant uropathogenic E.coli .One hundred clinical strains were isolated from patients with chronic urinary tract infections (UTIs), these isolates were identified by the Api identification systems. The antimicrobial susceptibility tests were determined by Kirby-Bauer method, all of them were sensitive to Imipenem (Imp). Ten strains were chosen for the present study, they were resistant to Ampicillin (Amp), Amoxicillin (Amo), Carbenicillin (Cb), Ticarcillin (Tic), Azlocillin (Azl), Amoxicillin\ Potassium Clavulanate {Augmentin(Amc)}, (Amo\CA), Ticarcillin\ Potassium Clavulanate {Timentin} (Tic\CA) ,Cefazolin (Cfo) ,Cefaloridin (Cfr), Cefamandol, (Cfm),Cefoxitin, Ceftazidime (Cfz), Cefixime   (Cxm), Cefoperazone( Cfp) and Aztreonam (Atm), also resistant to other antibiotics, Tetracycline(Tc),Cloramphenicol(Cm),Gentamycin(G),Amikacin (Amk), Ciprofloxacin (Cip) and Trimethoprim. 50% of the isolates were resistant to Nalidixic acid and Rifampicin. The minimum inhibitory concentrations of Cefamandol and Ceftazidime were determined, by tube method. Transfer of plasmids were done by direct conjugation test to sensitive standard E.coli ,cell free β- lactamases were prepared and detected by macro-iodometric method. The activity of each cell free ß– lactamases extract against Cfm and Cfz were determined by disks diffusion method (microbiological Masuda method). Excellent activities were obtained against these strains when Cfm and Cfz, combined with CA, therefore complete zones of inhibition were obtained indicated the prevalence of extended spectrum β- lactamases in E.coli. The stability of Cfm and Cfz in the presence of CA were useful in the treatment of chronic urinary tract infections caused by multiresistant β- lactamase (ESBL) producer E.coli.

【 授权许可】

CC BY|CC BY-ND|CC BY-NC|CC BY-NC-ND