Biological synthesis of silver nanoparticles by cell-free extract of Polysiphonia algae and their anticancer activity against breast cancer MCF-7 cell lines

Micro & nano letters

article

Azam Moshfegh¹  Amir Jalali²  Ali Salehzadeh³  Amin Sadeghi Jozani¹ 

[1] Department of Biology, Lahijan Branch, Islamic Azad University;Department of Biology, Faculty of Science, Arak University;Department of Biology, Rasht Branch, Islamic Azad University

关键词: nanoparticles;    cancer;    scanning electron microscopy;    nanofabrication;    transmission electron microscopy;    surface plasmon resonance;    ultraviolet spectra;    biomedical materials;    nanomedicine;    X-ray chemical analysis;    cellular biophysics;    toxicology;    visible spectra;    antibacterial activity;    particle size;    silver;    Fourier transform infrared spectra;    microorganisms;    biological synthesis;    silver nanoparticles;    polysiphonia algae;    anticancer activity;    breast cancer MCF-7 cell line;    green nanomaterial production techniques;    medicinal applications;    biocompatibility;    toxic by-products;    silver nitrate solutions;    yellow-brown colour formation;    surface plasmon resonance;    green synthesised AgNPs;    scanning electron microscopy;    transmission electron microscopy images;    spherical AgNPs;    synthesised nanoparticles;    anticancer AgNPs;    cancerous cells;    cell-free extract;    ultraviolet-visible spectroscopy;    energy-dispersive X-ray analysis;    reducing agent;    Fourier-transform infrared spectroscopy;    FTIR spectroscopy;    MTT assay;    flow cytometry;    inhibitory activity;    filamentous red alga Polysiphonia extract;    size 5.0 nm to 25.0 nm;    wavelength 420.0 nm;    Ag;   

DOI  :  10.1049/mnl.2018.5260

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Laryngoscopy and tracheal intubation are considered the most invasive stimuli in anesthesia. They provoked cardiovascular responses that include hypertension, tachycardia and dysrhythmias. Various pharmacological approaches have been used to blunt or attenuate such pressor responses. The present study was designed to evaluate the effect of medazolom, lignocaine and propranolol as a valuable adjuvant to fentanyl in attenuating hemodynamic responses to endotracheal intubation in normotensive patients. Thirty two patient with physical status I or II according to the score of American Society of Anesthesiologist (ASA), scheduled for elective surgery under standard general anesthesia, were randomly allocated into four groups (8 patients in each group), assigned as F, M, L and P groups. Each patient in the four groups received 1 µg/kg i.v fentanyl. Patients in groups M, L and P are treated with 0.2 mg/kg i.v medazolam, 1.5mg/kg i.v lignocaine and 0.01mg/kg i.v propranolol respectively. Induction of anesthesia was then accomplished with 2mg/kg thiopental sodium followed by1.5mg/kg succinylcholine. Tracheal intubation was performed 2 minutes after induction of anesthesia. Heart rate, systolic blood pressure, diastolic blood pressure, mean arterial pressure and rate pressure product were measured before induction, after induction and at 2, 4, 6 and 8 minutes after intubation. The results indicated no significant variation in the hemodynamic pressor response in all four groups with tracheal intubation. In conclusion, a minimum effective dose of i.v pre-medications (fentanyl, medazolom, lignocaine and propranolol) were found to be individually successful in attenuating and providing a reliable control of all hemodynamic response changes accompanied the process of laryngoscopy and intubation. Therefore, all are proved effective premedication and no one being superior.

【 授权许可】

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