Chitosan–heparin functionalised magnetic nanoparticles for the magnetic recovery of Aspergillus niger lipase enzyme

Micro & nano letters

article

Ana Karina Pérez-Guzmán¹  Anna Ilyina¹  Alejandro Zugasti-Cruz¹  Jose Daniel García-García¹  Elda Patricia Segura-Ceniceros¹  Jose Luis Martínez-Hernández¹  Rodolfo Ramos-González² 

[1] Universidad Autónoma de Coahuila;CONACYT – Universidad Autónoma de Coahuila

关键词: nanoparticles;    microorganisms;    nanomedicine;    magnetic particles;    enzymes;    nanomagnetics;    biochemistry;    nanocomposites;    biomedical materials;    X-ray diffraction;    nanofabrication;    biotechnology;    molecular biophysics;    Fourier transform infrared spectra;    precipitation (physical chemistry);    magnetic nanoparticles;    magnetic recovery;    MNPH;    coprecipitation method;    recovered lipase;    chitosan–heparin;    Aspergillus niger lipase enzyme;    Fourier transform infrared spectroscopy;    X-ray diffraction;    alternating gradient magnetometry;    Plackett–Burman design;    enzymatic activity;    ionic strength;    sodium dodecyl sulphate;    nanostructured system;    temperature 4.0 degC;    temperature 25.0 degC;   

DOI  :  10.1049/mnl.2018.5490

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

This work focuses on the obtention of magnetic nanoparticles functionalised with heparin (MNPH) and their interaction with Aspergillus niger lipase enzyme. The MNPH were obtained by the coprecipitation method, its characterisation was carried out by Fourier transform infrared spectroscopy, X-ray diffraction, and alternating gradient magnetometry. Functionalisation with heparin was carried out by ultrasound at various times, later the absorption of the enzyme was carried out with two temperatures 4 and 25°C at different concentrations of lipase. For the recovery of the enzyme, a Plackett–Burman design was used, which indicates that the recovered lipase maintains its enzymatic activity when the ionic strength is lower, it was characterised by sodium dodecyl sulphate-page with which it is demonstrated that it is possible to recover the lipase enzyme through the designed nanostructured system.

【 授权许可】

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