Highly sensitive NADH detection by utilising an aluminium hydroxide/iron hydroxide/MWCNTs nanocomposite film-modified electrode

Micro & nano letters

article

Yu Wang¹  Lingling Yin²  Xia Li¹  Ran Shang¹  Xiangli Yang¹  Xiaoyan Zhou¹  Yen Chen¹ 

[1] School of Resources and Environmental Engineering, Shandong Agriculture and Engineering University;Shandong Provincial Veterinary Medicine Supervision Institute/Shandong Provincial Key Laboratory of Quality Safety Monitoring and Risk Assessment for Livestock and Poultry Products

关键词: biochemistry;    nanobiotechnology;    molecular biophysics;    oxidation;    nanocomposites;    nanofabrication;    nanomedicine;    electrochemical sensors;    biosensors;    gold;    voltammetry (chemical analysis);    enzymes;    nanoparticles;    amperometric sensors;    electrochemical electrodes;    nanosensors;    electrocatalysis;    multi-wall carbon nanotubes;    aluminium compounds;    iron compounds;    thin films;    NADH-producing enzymes;    highly sensitive NADH detection;    glassy carbon electrode;    dihydro-nicotinamide adenine dinucleotide detection;    boosted electron transfer;    low-detection limit;    sensitive ethanol biosensor;    ethanol sensitivity;    aluminium hydroxide-iron hydroxid-MWCNTs nanocomposite film-modified electrode;    electrocatalytic response;    wide linear concentration range;    alcohol dehydrogenase;    voltage 0.15 V;    Ag-AgCl;    AlOH-FeOH-C;   

DOI  :  10.1049/mnl.2020.0073

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Glassy carbon electrode (GCE) modified with aluminium hydroxide/iron hydroxide/multi-walled carbon nanotubes (AH/IH/MWCNTs) composites has been prepared by a simple method and applied for dihydro-nicotinamide adenine dinucleotide (NADH) detection. AH/IH can not only accelerate electron transfer but also electrostatically interact with the phosphate groups of NADH through iron hydroxide to improve the sensitivity of the sensor. Meanwhile, MWCNTs served as a bonding agent to provide a built-in conductor, which resulted in boosted electron transfer at the interface. Compared with the GCE, MWCNTs–GCE, and AH/MWCNTs–GCE, the AH/IH/MWCNTs–GCE exhibited an extraordinary electrocatalytic response towards NADH, with a wide linear concentration range from 0.5 to 220 μM with a low-detection limit of 0.30 μM, at a comparatively low potential (+0.15 V versus Ag/AgCl). Moreover, alcohol dehydrogenase was used as a model system for the design of a sensitive ethanol biosensor. The resulting biosensor exhibited an ethanol sensitivity of 9 μA/mM, a concentration range of 20–400 μM, and a detection limit of 5 μM. These results demonstrate the potential of the AH/IH/MWCNTs nanocomposite film for biosensors in combination with NADH-producing enzymes.

【 授权许可】

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