Analysis of vaginal and endometrial microbiota communities in infertile women with a history of repeated implantation failure

Reproductive medicine and biology

article

Takuhiko Ichiyama¹  Keiji Kuroda²  Yoko Nagai⁴  Daichi Urushiyama⁶  Motoharu Ohno¹  Takashi Yamaguchi¹  Motoi Nagayoshi¹  Yoshiyuki Sakuraba⁴  Fumio Yamasaki⁷  Kenichiro Hata⁵  Shingo Miyamoto⁶  Atsuo Itakura²  Satoru Takeda²  Atsushi Tanaka¹ 

[1] Saint Mother Obstetrics and Gynecology Clinic and Institute for Assisted Reproductive Technologies;Department of Obstetrics and Gynecology, Juntendo University School of Medicine;Center for Reproductive Medicine and Implantation Research, Sugiyama Clinic Shinjuku;Varinos Inc.;Department of Maternal-Fetal Biology, National Research Institute for Child Health and Development;Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University;Division of Pathology, Japan Community Health Care Organization, Saga Central Hospital

关键词: 16S rRNA;    bacterial vaginosis;    dysbiosis;    microbiome;    microbiota;    repeated implantation failure;   

DOI  :  10.1002/rmb2.12389

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Purpose To identify specific bacterial communities in vaginal and endometrial microbiotas as biomarkers of implantation failure by comprehensively analyzing their microbiotas using next-generation sequencing. Methods We investigated α- and β-diversities of vaginal and endometrial microbiotas using 16S rRNA gene sequencing and compared their profiles between 145 women with repeated implantation failure (RIF) and 21 controls who lacked the factors responsible for implantation failure with a high probability of being healthy and fertile to identify specific bacteria that induce implantation failure. Results The endometrial microbiotas had higher α-diversities than did the vaginal microbiotas ( P  < .001). The microbiota profiles showed that vaginal and endometrial samples in RIF patients had significantly higher levels of 5 and 14 bacterial genera, respectively, than those in controls. Vaginal Lactobacillus rates in RIF patients were significantly lower at 76.4 ± 38.9% compared with those of the controls at 91.8 ± 22.7% ( P  = .018), but endometrial Lactobacillus rates did not significantly differ between the RIF patients and controls (56.2 ± 36.4% and 58.8 ± 37.0%, respectively, P  = .79). Conclusions Impaired microbiota communities containing specific bacteria in both the endometrium and vagina were associated with implantation failure. The vaginal Lactobacillus rates, but not the endometrial, may be a biomarker for RIF.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND