Characterization of the Vaginal DNA Virome in Health and Dysbiosis

Viruses

Thor Haahr¹  Peter Humaidan¹  WitoldPiotr Kot²  ThomasDyrmann Leser³  JørgenSkov Jensen⁴  RasmusRiemer Jakobsen⁵  Ling Deng⁵  DennisSandris Nielsen⁵  JosueLeonardo Castro-Mejia⁵ 

[1] Department of Clinical Medicine, Aarhus University, DK-8200 Aarhus, Denmark;Department of Environmental Sciences, Aarhus University, Risø, 4000 Roskilde, Denmark;Human Health, Innovation, Chr. Hansen A/S, 10-12 Boege Allé, DK-2970 Hoersholm, Denmark;Research Unit for Reproductive Microbiology, Statens Serum Institut, DK-2300 Copenhagen, Denmark;Section of Microbiology and Fermentation, Department of Food Science, Faculty of Science, University of Copenhagen, DK-1958 Copenhagen, Denmark;

关键词: vaginal microbiome;    vaginal virome;    bacteriophages;    bacterial vaginosis;    dysbiosis;   

DOI  :  10.3390/v12101143

来源: DOAJ

【 摘 要 】

Bacterial vaginosis (BV) is characterized by a reduction in Lactobacillus (L.) spp. abundance and increased abundance of facultative anaerobes, such as Gardnerella spp. BV aetiology is not fully understood; however, bacteriophages could play a pivotal role in the perturbation of the vaginal bacterial community. We investigated the vaginal viral community, including bacteriophages and the association to the bacterial community and BV-status. Vaginal samples from 48 patients undergoing IVF treatment for non-female factor infertility were subjected to metagenomic sequencing of purified virus-like particles. The vaginal viral community was characterized and correlated with the BV-status by Nugent score, bacterial community, structure, and the presence of key vaginal bacterial species. The majority of identified vaginal viruses belonged to the class of double-stranded DNA bacteriophages, with eukaryotic viruses constituting 4% of the total reads. Clear links between the viral community composition and BV (q = 0.006, R = 0.26) as well as the presence of L. crispatus (q = 0.001, R = 0.43), L. iners, Gardnerella spp., and Atopobium vaginae were found (q < 0.002, R > 0.15). The eukaryotic viral community also correlated with BV-status (q = 0.018, R = 0.20). In conclusion, the vaginal virome was clearly linked with bacterial community structure and BV-status.

【 授权许可】

Unknown