In vitro maturation of human immature oocytes for fertility preservation and research material

Reproductive medicine and biology

article

Hiromitsu Shirasawa¹  Yukihiro Terada¹ 

[1] Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine

关键词: assisted reproductive technology;    cryopreservation;    fertility preservation;    in vitro maturation;    oncofertility;   

DOI  :  10.1002/rmb2.12042

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Aim In recent years, the importance of fertility preservation (FP) has increased. In vitro maturation (IVM), an important technique in FP, has started to be used in the clinic, but controversies persist regarding this technique. Here, a survey of IVM for FP is provided. Methods Based on a literature review, the applications of FP, methods of FP, IVM of oocytes that had been collected in vivo and ex vivo, maturation of oocytes after IVM for FP, cryopreservation of oocytes for FP, explanation of the procedures to patients, and recent research on FP using IVM were investigated. Results Although IVM for FP remains controversial, the application of FP is expected to expand. Depending on the age and disease status of the patient, various methods of oocyte collection and ovarian stimulation, as well as various needle types and aspiration pressures, have been reported. The maturation rate of IVM in FP ranges widely and requires optimization in the future. In regard to cryopreservation for matured oocytes, the vitrification method is currently recommended. Conclusion Regarding FP for patients with cancer, the treatment of cancer is prioritized; thus, the time and use of medicines are often constrained. As several key points regarding IVM remain unclear, well-designed and specific counseling for patients is necessary.

【 授权许可】

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