期刊论文详细信息
Reproductive medicine and biology
Non-esterified fatty acid-associated ability of follicular fluid to support porcine oocyte maturation and development
article
Kaori Ogawa1  Nobuhiko Itami1  Manami Ueda1  Kazuki Kansaku1  Koumei Shirasuna1  Takehito Kuwayama1  Hisataka Iwata1 
[1] Department of Animal Science, Tokyo University of Agriculture
关键词: fatty acids;    follicular fluid;    granulosa cells;    mitochondria;    oocytes;   
DOI  :  10.1002/rmb2.12084
学科分类:工业工程学
来源: Wiley
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【 摘 要 】

Purpose The effect of supplementing maturation medium with follicular fluid (FF) was examined according to its non-esterified fatty acid (NEFA) content or with a fatty acid mixture (FA-Mix) on the developmental competence of oocytes, as well as the mitochondrial quality and quantity in the oocytes and cumulus cells. Method Porcine oocytes from a slaughterhouse were used. Results The FF or FA-Mix in maturation medium increased the lipid content in both the oocytes and the cumulus cells, but the adenosine triphosphate content was differentially affected. The FF supplementation increased the mitochondrial DNA copy number, survival of cumulus cells, and rate of oocyte development to the blastocyst stage, whereas the FA-Mix supplementation did not show these effects. The expression levels of GPC4 , PFKP , PRDX3 , and TFAM in the cumulus cells increased after FF supplementation, but the expression of GJA1 decreased, compared with the cells that were cultured without FF. Conclusion Adding FF and FA-Mix to the maturation medium increased the lipid content in the oocytes and cumulus cells. The effects of FF on the cumulus cells and oocytes were not observed after FA-Mix supplementation, indicating that the concentration of the NEFAs in the FF are closely associated with an ability to support oocyte maturation and the metabolism of cumulus cells and oocytes.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND   

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