Potassium bromate disrupts mitochondrial distribution within murine oocytes during in vitro maturation

Reproductive medicine and biology

article

Kenichi Yamada¹  Yuuki Hiradate¹  Mei Goto¹  Chiho Nishiyama¹  Kenshiro Hara¹  Hiroaki Yoshida²  Kentaro Tanemura¹ 

[1] Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University;Sendai ART Clinic

关键词: aged oocytes;    antioxidant;    in vitro maturation;    mitochondria;    oxidizing agents;   

DOI  :  10.1002/rmb2.12079

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Purpose As disturbed mitochondrial distribution is thought to be a cause of the aging of oocytes, it was investigated whether oxidizing agents exert harmful effects on nuclear maturation and mitochondrial cluster formation in murine oocytes and whether antioxidants could rescue such harmful effects in vitro. Methods Oocytes were obtained from female Institute of Cancer Research mice 48 h after an intraperitoneal injection of 7.5 IU pregnant mare serum gonadotropin. The oocytes were cultured with potassium bromate, an oxidizing agent, in the presence or absence of the antioxidant, resveratrol. After 12 h, the nuclear phases and mitochondrial distribution were observed. Results Significantly decreased rates of metaphase II (MII) oocytes were observed with 750 μM and 1000 μM of potassium bromate, while a significant increase in abnormal mitochondrial clusters was induced at 500 μM, 750 μM, and 1,000 μM. The addition of 10 μM or 20 μM resveratrol improved both MII maturity and the cluster formation rates in the presence of potassium bromate. Conclusions The addition of potassium bromate reduced MII maturity rates and induced abnormal mitochondrial cluster formation. This effect was alleviated by the antioxidant, resveratrol. The in vitro model used herein is useful for investigating the functions of antioxidants in the aging of oocytes.

【 授权许可】

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